Abstract
Telomerase contains an essential RNA, which includes the template sequence copied by the reverse transcription action of telomerase into telomeric DNA. Using phylogenetic comparison, we identified seven conserved sequences in telomerase RNAs from Kluyveromyces budding yeasts. We show that two of these sequences, CS3 and CS4, are essential for normal telomerase function and can base-pair to form a putative long-range pseudoknot. Disrupting this base-pairing was deleterious to cell growth, telomere maintenance, and telomerase activity. Restoration of the base-pairing potential alleviated these phenotypes. Mutating this pseudoknot caused a novel mode of shifting of the boundaries of the RNA template sequence copied by telomerase. A phylogenetically derived model of yeast TER structure indicates that these RNAs can form two alternative predicted core conformations of similar stability: one brings the CS3/CS4 pseudoknot spatially close to the template; in the other, CS3 and CS4 move apart and the conformation of the template is altered. We propose that such disruption of the pseudoknot, and potentially the predicted telomerase RNA conformation, affects polymerization to cause the observed shifts in template usage.
| Original language | American English |
|---|---|
| Pages (from-to) | 1779-1788 |
| Number of pages | 10 |
| Journal | Genes and Development |
| Volume | 17 |
| Issue number | 14 |
| DOIs | |
| State | Published - 15 Jul 2003 |
| Externally published | Yes |
Keywords
- Telomerase RNA pseudoknot
- Telomerase RNA secondary structure
- Telomerase template miscopying
- Telomerase, yeast telomerase RNA