A novel spontaneous mutation in the tap2 gene unravels its role in macrophage survival

Antonio Lapenna, Ibrahim Omar, Michael Berger*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

We report a new mouse strain with a single point mutation in the type 2 transporter associated with antigen processing (TAP2). This strain randomly arose in one of our C57BL/6J mouse colonies and was initially discovered because of the lack of CD8+T cells in the periphery. Following our observation, we subsequently revealed a lack of cell surface MHC-I expression, derived from TAP2 protein deficiency. Our strain, named eightless, has a C to T substitution in exon 5 resulting in a glutamine to stop codon substitution at position 285 in the TAP2 protein. Interestingly, in addition to the expected lack of CD8+T cell phenotype, eightless mice have a diminished number of macrophages in their peritoneum. Moreover, following peritoneal inflammation, elicited eightless macrophages showed impaired survival both in vivo and ex vivo. Our study describes the first ever TAP2 complete knockout mouse strain and provides a possible explanation for why patients with TAP2 deficiency syndrome present clinical manifestations that would suggest a phagocyte defect rather than a lack of CD8+T cells.

Original languageAmerican English
Pages (from-to)432-443
Number of pages12
JournalImmunology
Volume150
Issue number4
DOIs
StatePublished - 1 Apr 2017

Bibliographical note

Publisher Copyright:
© 2016 John Wiley & Sons Ltd.

Keywords

  • Immunodeficiency diseases
  • Major histocompatibility complex
  • T cells

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