Abstract
A simple system for cutting dendrites of single neurons without damaging the viability of the cell is described. The system utilizes a rapidly vibrating (100 Hz) micropipette (<1 μm tip diameter), which is dragged under direct visual control across the dendrite of a selected neuron. We used this system in a thin slice preparation to dissect the apical dendrites of rat hippocampal CA1 pyramidal cells. We then used the patch-clamp technique in the whole-cell configuration to record from the isolated somata of these cells and to inject a dye into them. Both a functional and an anatomical disconnection of the dendrites from their somata were verified. Copyright (C) 2000 Elsevier Science B.V.
Original language | English |
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Pages (from-to) | 119-122 |
Number of pages | 4 |
Journal | Journal of Neuroscience Methods |
Volume | 98 |
Issue number | 2 |
DOIs | |
State | Published - 1 Jun 2000 |
Keywords
- Dendrites
- Hippocampus
- Isolated-soma
- Micro-dissection
- Piezoelectric
- Space-clamp