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A protective human monoclonal IgA antibody produced in vitro: Anti‐pneumococcal antibody engendered by Epstein‐Barr virus‐immortalized cell line

  • Michael Steinitz*
  • , Sara Tamir
  • , Mina Ferne
  • , Ada Goldfarb
  • *Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

Human lymphocytes that produce anti‐pneumococcal antibodies were separated and immortalized by Epstein‐Barr virus and then cloned. One clone (NAD‐Sel) produces an IgA, x antibody which is specific for the polysaccharides of type 8 pneumococcus, while not reactive with any of the polysaccharides derived from 24 other pneumococcal strains. The antibody, which is present in the cell supernatant as monomer and polymer, binds to protein A and does not fix complement. When incubated in vitro with type 8 pneumococci, it induces direct killing and increases the opsonization of these bacteria by mouse macrophages.

Original languageEnglish
Pages (from-to)187-193
Number of pages7
JournalEuropean Journal of Immunology
Volume16
Issue number2
DOIs
StatePublished - 1986
Externally publishedYes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

  1. SDG 3 - Good Health and Well-being
    SDG 3 Good Health and Well-being

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