TY - JOUR
T1 - A putative ATP-activated Na+ channel involved in sperm-induced fertilization
AU - Kupitz, Yuval
AU - Atlas, Daphne
PY - 1993
Y1 - 1993
N2 - Extracellular application of adenosine triphosphate (ATP) to defolliculated Xenopus laevis oocytes activated a saturating inward current with a maximal amplitude Emax of 2.4 ± 0.2 microamperes and an apparent Michaelis constant of 197.6 micromolar. The current was carried predominantly by sodium ions and potently inhibited by amiloride, guanosine triphosphate (GTP), and its nonhydrolyzable analogs guanosine 5′-[β,γ-imido] triphosphate (GppNHp) and guanosine 5′-O-(3-thiotriphosphate). Likewise, in vitro fertilization using mature eggs and Xenopus sperm was inhibited by amiloride, GTP, and GppNHp. Hence, an ATP receptor on the egg membrane may be the recipient target for ATP originating in sperm, suggesting that an ATP-induced increase in sodium permeability mediates the initial sperm to egg signal in the fertilization process.
AB - Extracellular application of adenosine triphosphate (ATP) to defolliculated Xenopus laevis oocytes activated a saturating inward current with a maximal amplitude Emax of 2.4 ± 0.2 microamperes and an apparent Michaelis constant of 197.6 micromolar. The current was carried predominantly by sodium ions and potently inhibited by amiloride, guanosine triphosphate (GTP), and its nonhydrolyzable analogs guanosine 5′-[β,γ-imido] triphosphate (GppNHp) and guanosine 5′-O-(3-thiotriphosphate). Likewise, in vitro fertilization using mature eggs and Xenopus sperm was inhibited by amiloride, GTP, and GppNHp. Hence, an ATP receptor on the egg membrane may be the recipient target for ATP originating in sperm, suggesting that an ATP-induced increase in sodium permeability mediates the initial sperm to egg signal in the fertilization process.
UR - http://www.scopus.com/inward/record.url?scp=0027292154&partnerID=8YFLogxK
U2 - 10.1126/science.8392753
DO - 10.1126/science.8392753
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C2 - 8392753
AN - SCOPUS:0027292154
SN - 0036-8075
VL - 261
SP - 484
EP - 486
JO - Science
JF - Science
IS - 5120
ER -