A putative HCO3/- transporter in the cyanobacterium Synechococcus sp. strain PCC 7942

David J. Bonfil, Michal Ronen-Tarazi, Dieter Sültemeyer, Judy Lieman-Hurwitz, Daniella Schatz, Aaron Kaplan*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

57 Scopus citations

Abstract

Cyanobacteria possess an inducible mechanism which enables them to concentrate inorganic carbon (Ci) within the cells. An inactivation library was used to raise the high-CO2-requiring mutant of Synechococcus PCC 7942, IL-2, impaired in HCO3/- transport. Analysis of the relevant genomic DNA detected several modifications, probably due to the single crossover recombination, leading to inactivation of ORF467 (designated ictB) in IL-2. IctB contains 10 trans-membrane regions and is homologous to several transport-related proteins from various organisms. Kinetic analyses of HCO3/- uptake in the wild type and IL-2 suggested the presence of two or three HCO3/- carriers exhibiting different affinities to HCO3/-.

Original languageEnglish
Pages (from-to)236-240
Number of pages5
JournalFEBS Letters
Volume430
Issue number3
DOIs
StatePublished - 3 Jul 1998

Keywords

  • CO-concentrating mechanism
  • Crossover
  • Cyanobacteria
  • HCO/ uptake
  • IctB

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