Abstract
Cyanobacteria possess an inducible mechanism which enables them to concentrate inorganic carbon (Ci) within the cells. An inactivation library was used to raise the high-CO2-requiring mutant of Synechococcus PCC 7942, IL-2, impaired in HCO3/- transport. Analysis of the relevant genomic DNA detected several modifications, probably due to the single crossover recombination, leading to inactivation of ORF467 (designated ictB) in IL-2. IctB contains 10 trans-membrane regions and is homologous to several transport-related proteins from various organisms. Kinetic analyses of HCO3/- uptake in the wild type and IL-2 suggested the presence of two or three HCO3/- carriers exhibiting different affinities to HCO3/-.
Original language | English |
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Pages (from-to) | 236-240 |
Number of pages | 5 |
Journal | FEBS Letters |
Volume | 430 |
Issue number | 3 |
DOIs | |
State | Published - 3 Jul 1998 |
Keywords
- CO-concentrating mechanism
- Crossover
- Cyanobacteria
- HCO/ uptake
- IctB