A putative HCO3/- transporter in the cyanobacterium Synechococcus sp. strain PCC 7942

David J. Bonfil; Michal Ronen-Tarazi; Dieter Sültemeyer; Judy Lieman-Hurwitz; Daniella Schatz; Aaron Kaplan

doi:10.1016/S0014-5793(98)00662-0

A putative HCO₃/^- transporter in the cyanobacterium Synechococcus sp. strain PCC 7942

David J. Bonfil
, Michal Ronen-Tarazi
, Dieter Sültemeyer
, Judy Lieman-Hurwitz
, Daniella Schatz
, Aaron Kaplan^*

^*Corresponding author for this work

Alexander Silberman Institute of Life Sciences

Research output: Contribution to journal › Article › peer-review

58 Scopus citations

Abstract

Cyanobacteria possess an inducible mechanism which enables them to concentrate inorganic carbon (Ci) within the cells. An inactivation library was used to raise the high-CO₂-requiring mutant of Synechococcus PCC 7942, IL-2, impaired in HCO₃/^- transport. Analysis of the relevant genomic DNA detected several modifications, probably due to the single crossover recombination, leading to inactivation of ORF467 (designated ictB) in IL-2. IctB contains 10 trans-membrane regions and is homologous to several transport-related proteins from various organisms. Kinetic analyses of HCO₃/^- uptake in the wild type and IL-2 suggested the presence of two or three HCO₃/^- carriers exhibiting different affinities to HCO₃/^-.

Original language	English
Pages (from-to)	236-240
Number of pages	5
Journal	FEBS Letters
Volume	430
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(98)00662-0
State	Published - 3 Jul 1998

Keywords

CO-concentrating mechanism
Crossover
Cyanobacteria
HCO/ uptake
IctB

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10.1016/S0014-5793(98)00662-0

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title = "A putative HCO3/- transporter in the cyanobacterium Synechococcus sp. strain PCC 7942",

abstract = "Cyanobacteria possess an inducible mechanism which enables them to concentrate inorganic carbon (Ci) within the cells. An inactivation library was used to raise the high-CO2-requiring mutant of Synechococcus PCC 7942, IL-2, impaired in HCO3/- transport. Analysis of the relevant genomic DNA detected several modifications, probably due to the single crossover recombination, leading to inactivation of ORF467 (designated ictB) in IL-2. IctB contains 10 trans-membrane regions and is homologous to several transport-related proteins from various organisms. Kinetic analyses of HCO3/- uptake in the wild type and IL-2 suggested the presence of two or three HCO3/- carriers exhibiting different affinities to HCO3/-.",

keywords = "CO-concentrating mechanism, Crossover, Cyanobacteria, HCO/ uptake, IctB",

author = "Bonfil, \{David J.\} and Michal Ronen-Tarazi and Dieter S{\"u}ltemeyer and Judy Lieman-Hurwitz and Daniella Schatz and Aaron Kaplan",

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doi = "10.1016/S0014-5793(98)00662-0",

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T1 - A putative HCO3/- transporter in the cyanobacterium Synechococcus sp. strain PCC 7942

AU - Bonfil, David J.

AU - Ronen-Tarazi, Michal

AU - Sültemeyer, Dieter

AU - Lieman-Hurwitz, Judy

AU - Schatz, Daniella

AU - Kaplan, Aaron

PY - 1998/7/3

Y1 - 1998/7/3

N2 - Cyanobacteria possess an inducible mechanism which enables them to concentrate inorganic carbon (Ci) within the cells. An inactivation library was used to raise the high-CO2-requiring mutant of Synechococcus PCC 7942, IL-2, impaired in HCO3/- transport. Analysis of the relevant genomic DNA detected several modifications, probably due to the single crossover recombination, leading to inactivation of ORF467 (designated ictB) in IL-2. IctB contains 10 trans-membrane regions and is homologous to several transport-related proteins from various organisms. Kinetic analyses of HCO3/- uptake in the wild type and IL-2 suggested the presence of two or three HCO3/- carriers exhibiting different affinities to HCO3/-.

AB - Cyanobacteria possess an inducible mechanism which enables them to concentrate inorganic carbon (Ci) within the cells. An inactivation library was used to raise the high-CO2-requiring mutant of Synechococcus PCC 7942, IL-2, impaired in HCO3/- transport. Analysis of the relevant genomic DNA detected several modifications, probably due to the single crossover recombination, leading to inactivation of ORF467 (designated ictB) in IL-2. IctB contains 10 trans-membrane regions and is homologous to several transport-related proteins from various organisms. Kinetic analyses of HCO3/- uptake in the wild type and IL-2 suggested the presence of two or three HCO3/- carriers exhibiting different affinities to HCO3/-.

KW - CO-concentrating mechanism

KW - Crossover

KW - Cyanobacteria

KW - HCO/ uptake

KW - IctB

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A putative HCO₃/^- transporter in the cyanobacterium Synechococcus sp. strain PCC 7942

Abstract

Keywords

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