A Rapid and Efficient Building Block Approach for Click Cyclization of Peptoids

Mamidi Samarasimhareddy, Mai Shamir, Deborah E. Shalev, Mattan Hurevich*, Assaf Friedler

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Cyclic peptide-peptoid hybrids possess improved stability and selectivity over linear peptides and are thus better drug candidates. However, their synthesis is far from trivial and is usually difficult to automate. Here we describe a new rapid and efficient approach for the synthesis of click-based cyclic peptide-peptoid hybrids. Our methodology is based on a combination between easily synthesized building blocks, automated microwave assisted solid phase synthesis and bioorthogonal click cyclization. We proved the concept of this method using the INS peptide, which we have previously shown to activate the HIV-1 integrase enzyme. This strategy enabled the rapid synthesis and biophysical evaluation of a library of cyclic peptide-peptoid hybrids derived from HIV-1 integrase in high yield and purity. The new cyclic hybrids showed improved biological activity and were significantly more stable than the original linear INS peptide.

Original languageAmerican English
Article number405
JournalFrontiers in Chemistry
Volume8
DOIs
StatePublished - 19 May 2020

Bibliographical note

Publisher Copyright:
© Copyright © 2020 Samarasimhareddy, Shamir, Shalev, Hurevich and Friedler.

Keywords

  • click chemistry
  • peptide backbone modification
  • peptide cyclization
  • peptide-peptoid hybrids
  • peptides
  • peptoids

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