A rapid and sensitive ELISA for serum ferritin employing a fluorogenic substrate

A. M. Konijn*, R. Levy, G. Link, C. Hershko

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

60 Scopus citations

Abstract

A fluorescent enzyme-linked immunosorbent assay is described for the rapid measurement of serum ferritin. Increased sensitivity was achieved by using 4-methyl-umbelliferyl-β-d-galactopyranoside as the substrate for β-galactosidase coupled to the purified antiferritin antibody. Further enhancement of the specific antigen-antibody reaction was attained by the addition of 4% polyethylene glycol 6000 to the antiferritin-β-galactosidase conjugate. The procedure is performed in microELISA® plates. These modifications of the method permit the measurement of serum ferritin at concentrations ranging from 0.25 to 50 μg/liter with a coefficient of variation of 8% or less. The entire procedure is performed at ambient temperature and is completed within one working day. The cost of the assay is less than 10% of the immunoradiometric assay for serum ferritin.

Original languageAmerican English
Pages (from-to)297-307
Number of pages11
JournalJournal of Immunological Methods
Volume54
Issue number3
DOIs
StatePublished - 12 Nov 1982

Keywords

  • fluorescence ELISA
  • serum ferritin assay
  • β-galactosidase conjugate

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