TY - JOUR
T1 - A rapid and sensitive ELISA for serum ferritin employing a fluorogenic substrate
AU - Konijn, A. M.
AU - Levy, R.
AU - Link, G.
AU - Hershko, C.
PY - 1982/11/12
Y1 - 1982/11/12
N2 - A fluorescent enzyme-linked immunosorbent assay is described for the rapid measurement of serum ferritin. Increased sensitivity was achieved by using 4-methyl-umbelliferyl-β-d-galactopyranoside as the substrate for β-galactosidase coupled to the purified antiferritin antibody. Further enhancement of the specific antigen-antibody reaction was attained by the addition of 4% polyethylene glycol 6000 to the antiferritin-β-galactosidase conjugate. The procedure is performed in microELISA® plates. These modifications of the method permit the measurement of serum ferritin at concentrations ranging from 0.25 to 50 μg/liter with a coefficient of variation of 8% or less. The entire procedure is performed at ambient temperature and is completed within one working day. The cost of the assay is less than 10% of the immunoradiometric assay for serum ferritin.
AB - A fluorescent enzyme-linked immunosorbent assay is described for the rapid measurement of serum ferritin. Increased sensitivity was achieved by using 4-methyl-umbelliferyl-β-d-galactopyranoside as the substrate for β-galactosidase coupled to the purified antiferritin antibody. Further enhancement of the specific antigen-antibody reaction was attained by the addition of 4% polyethylene glycol 6000 to the antiferritin-β-galactosidase conjugate. The procedure is performed in microELISA® plates. These modifications of the method permit the measurement of serum ferritin at concentrations ranging from 0.25 to 50 μg/liter with a coefficient of variation of 8% or less. The entire procedure is performed at ambient temperature and is completed within one working day. The cost of the assay is less than 10% of the immunoradiometric assay for serum ferritin.
KW - fluorescence ELISA
KW - serum ferritin assay
KW - β-galactosidase conjugate
UR - http://www.scopus.com/inward/record.url?scp=0019994281&partnerID=8YFLogxK
U2 - 10.1016/0022-1759(82)90314-3
DO - 10.1016/0022-1759(82)90314-3
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C2 - 6816855
AN - SCOPUS:0019994281
SN - 0022-1759
VL - 54
SP - 297
EP - 307
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 3
ER -