Abstract
A fluorescent enzyme-linked immunosorbent assay is described for the rapid measurement of serum ferritin. Increased sensitivity was achieved by using 4-methyl-umbelliferyl-β-d-galactopyranoside as the substrate for β-galactosidase coupled to the purified antiferritin antibody. Further enhancement of the specific antigen-antibody reaction was attained by the addition of 4% polyethylene glycol 6000 to the antiferritin-β-galactosidase conjugate. The procedure is performed in microELISA® plates. These modifications of the method permit the measurement of serum ferritin at concentrations ranging from 0.25 to 50 μg/liter with a coefficient of variation of 8% or less. The entire procedure is performed at ambient temperature and is completed within one working day. The cost of the assay is less than 10% of the immunoradiometric assay for serum ferritin.
Original language | American English |
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Pages (from-to) | 297-307 |
Number of pages | 11 |
Journal | Journal of Immunological Methods |
Volume | 54 |
Issue number | 3 |
DOIs | |
State | Published - 12 Nov 1982 |
Keywords
- fluorescence ELISA
- serum ferritin assay
- β-galactosidase conjugate