A rapid and sensitive ELISA for serum ferritin employing a fluorogenic substrate

A. M. Konijn; R. Levy; G. Link; C. Hershko

doi:10.1016/0022-1759(82)90314-3

A rapid and sensitive ELISA for serum ferritin employing a fluorogenic substrate

A. M. Konijn^*, R. Levy, G. Link, C. Hershko

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

60 Scopus citations

Abstract

A fluorescent enzyme-linked immunosorbent assay is described for the rapid measurement of serum ferritin. Increased sensitivity was achieved by using 4-methyl-umbelliferyl-β-d-galactopyranoside as the substrate for β-galactosidase coupled to the purified antiferritin antibody. Further enhancement of the specific antigen-antibody reaction was attained by the addition of 4% polyethylene glycol 6000 to the antiferritin-β-galactosidase conjugate. The procedure is performed in microELISA^® plates. These modifications of the method permit the measurement of serum ferritin at concentrations ranging from 0.25 to 50 μg/liter with a coefficient of variation of 8% or less. The entire procedure is performed at ambient temperature and is completed within one working day. The cost of the assay is less than 10% of the immunoradiometric assay for serum ferritin.

Original language	English
Pages (from-to)	297-307
Number of pages	11
Journal	Journal of Immunological Methods
Volume	54
Issue number	3
DOIs	https://doi.org/10.1016/0022-1759(82)90314-3
State	Published - 12 Nov 1982

Keywords

fluorescence ELISA
serum ferritin assay
β-galactosidase conjugate

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10.1016/0022-1759(82)90314-3

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title = "A rapid and sensitive ELISA for serum ferritin employing a fluorogenic substrate",

abstract = "A fluorescent enzyme-linked immunosorbent assay is described for the rapid measurement of serum ferritin. Increased sensitivity was achieved by using 4-methyl-umbelliferyl-β-d-galactopyranoside as the substrate for β-galactosidase coupled to the purified antiferritin antibody. Further enhancement of the specific antigen-antibody reaction was attained by the addition of 4% polyethylene glycol 6000 to the antiferritin-β-galactosidase conjugate. The procedure is performed in microELISA{\textregistered} plates. These modifications of the method permit the measurement of serum ferritin at concentrations ranging from 0.25 to 50 μg/liter with a coefficient of variation of 8% or less. The entire procedure is performed at ambient temperature and is completed within one working day. The cost of the assay is less than 10% of the immunoradiometric assay for serum ferritin.",

keywords = "fluorescence ELISA, serum ferritin assay, β-galactosidase conjugate",

author = "Konijn, {A. M.} and R. Levy and G. Link and C. Hershko",

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T1 - A rapid and sensitive ELISA for serum ferritin employing a fluorogenic substrate

AU - Konijn, A. M.

AU - Levy, R.

AU - Link, G.

AU - Hershko, C.

PY - 1982/11/12

Y1 - 1982/11/12

N2 - A fluorescent enzyme-linked immunosorbent assay is described for the rapid measurement of serum ferritin. Increased sensitivity was achieved by using 4-methyl-umbelliferyl-β-d-galactopyranoside as the substrate for β-galactosidase coupled to the purified antiferritin antibody. Further enhancement of the specific antigen-antibody reaction was attained by the addition of 4% polyethylene glycol 6000 to the antiferritin-β-galactosidase conjugate. The procedure is performed in microELISA® plates. These modifications of the method permit the measurement of serum ferritin at concentrations ranging from 0.25 to 50 μg/liter with a coefficient of variation of 8% or less. The entire procedure is performed at ambient temperature and is completed within one working day. The cost of the assay is less than 10% of the immunoradiometric assay for serum ferritin.

AB - A fluorescent enzyme-linked immunosorbent assay is described for the rapid measurement of serum ferritin. Increased sensitivity was achieved by using 4-methyl-umbelliferyl-β-d-galactopyranoside as the substrate for β-galactosidase coupled to the purified antiferritin antibody. Further enhancement of the specific antigen-antibody reaction was attained by the addition of 4% polyethylene glycol 6000 to the antiferritin-β-galactosidase conjugate. The procedure is performed in microELISA® plates. These modifications of the method permit the measurement of serum ferritin at concentrations ranging from 0.25 to 50 μg/liter with a coefficient of variation of 8% or less. The entire procedure is performed at ambient temperature and is completed within one working day. The cost of the assay is less than 10% of the immunoradiometric assay for serum ferritin.

KW - fluorescence ELISA

KW - serum ferritin assay

KW - β-galactosidase conjugate

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A rapid and sensitive ELISA for serum ferritin employing a fluorogenic substrate

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