A recombinant fungal compound induces anti-proliferative and pro-apoptotic effects on colon cancer cells

Lili Nimri, Orly Spivak, Dana Tal, Dominik Schälling, Irena Peri, Lutz Graeve, Tomer M. Salame, Oded Yarden, Yitzhak Hadar, Betty Schwartz*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Finding intracellular pathways and molecules that can prevent the proliferation of colon cancer cells can provide significant bases for developing treatments for this disease. Ostreolysin (Oly) is a protein found in the mushroom Pleurotus ostreatus, and we have produced a recombinant version of this protein (rOly). We measured the viability of several colon cancer cells treated with rOly. Xenografts and syngeneic colon cancer cells were injected into in vivo mouse models, which were then treated with this recombinant protein. rOly treatment induced a significant reduction in viability of human and mouse colon cancer cells. In contrast, there was no reduction in the viability of normal epithelial cells from the small intestine. In the search for cellular targets of rOly, we showed that it enhances the anti-proliferative activity of drugs targeting cellular tubulin. This was accompanied by a reduction in the weight and volume of tumours in mice injected with rOly as compared to their respective control mice in two in vivo models. Our results advance the functional understanding of rOly as a potential anticancer treatment associated with pro-apoptotic activities preferentially targeting colon cancer cells.

Original languageAmerican English
Pages (from-to)28854-28864
Number of pages11
JournalOncotarget
Volume8
Issue number17
DOIs
StatePublished - 2017

Keywords

  • Colon cancer
  • Fungal
  • Microtubule
  • Pro-apoptotic
  • Recombinant ostreolysin

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