A relAS suppressor mutant allele of Bacillus subtilis which maps to relA and responds only to carbon limitation

Michal Gropp, Einat Eizenman, Gad Glaser, Walied Samarrai, Rivka Rudner*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

The histidine analog 3-amino-1,2,4-triazole (AT) was used for the selection of spontaneous AT-resistant revertants of a relA mutant of Bacillus subtilis. One of these revertants, L3, showed a unique phenotype; it did not respond to amino acid starvation, like the relA mutant, but it did respond to glucose starvation by the accumulation of (p)ppGpp, unlike its parent. Genetic analysis revealed that this suppressor mutant (relAS) allele mapped to the relA locus at 239° on the B. subtilis chromosome.

Original languageEnglish
Pages (from-to)91-96
Number of pages6
JournalGene
Volume140
Issue number1
DOIs
StatePublished - 1994

Keywords

  • (p)ppGpp
  • amino-acid starvation
  • amino-triazole
  • Stringent response
  • suppressor
  • transduction and transformation crosses

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