A relAS suppressor mutant allele of Bacillus subtilis which maps to relA and responds only to carbon limitation

Michal Gropp; Einat Eizenman; Gad Glaser; Walied Samarrai; Rivka Rudner

doi:10.1016/0378-1119(94)90736-6

A relA^S suppressor mutant allele of Bacillus subtilis which maps to relA and responds only to carbon limitation

Michal Gropp
, Einat Eizenman
, Gad Glaser
, Walied Samarrai
, Rivka Rudner^*

^*Corresponding author for this work

Institute for Medical Research Israel-Canada (IMRIC)

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

The histidine analog 3-amino-1,2,4-triazole (AT) was used for the selection of spontaneous AT-resistant revertants of a relA mutant of Bacillus subtilis. One of these revertants, L3, showed a unique phenotype; it did not respond to amino acid starvation, like the relA mutant, but it did respond to glucose starvation by the accumulation of (p)ppGpp, unlike its parent. Genetic analysis revealed that this suppressor mutant (relA^S) allele mapped to the relA locus at 239° on the B. subtilis chromosome.

Original language	English
Pages (from-to)	91-96
Number of pages	6
Journal	Gene
Volume	140
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(94)90736-6
State	Published - 1994

Keywords

(p)ppGpp
Stringent response
amino-acid starvation
amino-triazole
suppressor
transduction and transformation crosses

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10.1016/0378-1119(94)90736-6

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title = "A relAS suppressor mutant allele of Bacillus subtilis which maps to relA and responds only to carbon limitation",

abstract = "The histidine analog 3-amino-1,2,4-triazole (AT) was used for the selection of spontaneous AT-resistant revertants of a relA mutant of Bacillus subtilis. One of these revertants, L3, showed a unique phenotype; it did not respond to amino acid starvation, like the relA mutant, but it did respond to glucose starvation by the accumulation of (p)ppGpp, unlike its parent. Genetic analysis revealed that this suppressor mutant (relAS) allele mapped to the relA locus at 239° on the B. subtilis chromosome.",

keywords = "(p)ppGpp, Stringent response, amino-acid starvation, amino-triazole, suppressor, transduction and transformation crosses",

author = "Michal Gropp and Einat Eizenman and Gad Glaser and Walied Samarrai and Rivka Rudner",

year = "1994",

doi = "10.1016/0378-1119(94)90736-6",

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T1 - A relAS suppressor mutant allele of Bacillus subtilis which maps to relA and responds only to carbon limitation

AU - Gropp, Michal

AU - Eizenman, Einat

AU - Glaser, Gad

AU - Samarrai, Walied

AU - Rudner, Rivka

PY - 1994

Y1 - 1994

N2 - The histidine analog 3-amino-1,2,4-triazole (AT) was used for the selection of spontaneous AT-resistant revertants of a relA mutant of Bacillus subtilis. One of these revertants, L3, showed a unique phenotype; it did not respond to amino acid starvation, like the relA mutant, but it did respond to glucose starvation by the accumulation of (p)ppGpp, unlike its parent. Genetic analysis revealed that this suppressor mutant (relAS) allele mapped to the relA locus at 239° on the B. subtilis chromosome.

AB - The histidine analog 3-amino-1,2,4-triazole (AT) was used for the selection of spontaneous AT-resistant revertants of a relA mutant of Bacillus subtilis. One of these revertants, L3, showed a unique phenotype; it did not respond to amino acid starvation, like the relA mutant, but it did respond to glucose starvation by the accumulation of (p)ppGpp, unlike its parent. Genetic analysis revealed that this suppressor mutant (relAS) allele mapped to the relA locus at 239° on the B. subtilis chromosome.

KW - (p)ppGpp

KW - Stringent response

KW - amino-acid starvation

KW - amino-triazole

KW - suppressor

KW - transduction and transformation crosses

UR - https://www.scopus.com/pages/publications/0028345832

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AN - SCOPUS:0028345832

SN - 0378-1119

VL - 140

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EP - 96

JO - Gene

JF - Gene

IS - 1

ER -

A relA^S suppressor mutant allele of Bacillus subtilis which maps to relA and responds only to carbon limitation

Abstract

Keywords

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