A respiratory chain controlled signal transduction cascade in the mitochondrial intermembrane space mediates hydrogen peroxide signaling

Reactive oxygen species (ROS) such as hydrogen peroxide (H₂O₂) govern cellular homeostasis by inducing signaling. H₂O₂ modulates the activity of phosphatases and many other signaling molecules through oxidation of critical cysteine residues, which led to the notion that initiation of ROS signaling is broad and nonspecific, and thus fundamentally distinct from other signaling pathways. Here, we report that H₂O₂ signaling bears hallmarks of a regular signal transduction cascade. It is controlled by hierarchical signaling events resulting in a focused response as the results place the mitochondrial respiratory chain upstream of tyrosine-protein kinase Lyn, Lyn upstream of tyrosine-protein kinase SYK (Syk), and Syk upstream of numerous targets involved in signaling, transcription, translation,metabolism, and cell cycle regulation. The active mediators of H₂O₂ signaling colocalize as H₂O₂ induces mitochondria-associated Lyn and Syk phosphorylation, and a pool of Lyn and Syk reside in the mitochondrial intermembrane space. Finally, the same intermediaries control the signaling response in tissues and species responsive to H₂O₂ as the respiratory chain, Lyn, and Syk were similarly required for H₂O₂ signaling in mouse B cells, fibroblasts, and chicken DT40 B cells. Consistent with a broad role, the Syk pathway is coexpressed across tissues, is of early metazoan origin, and displays evidence of evolutionary constraint in the human. These results suggest that H₂O₂ signaling is under control of a signal transduction pathway that links the respiratory chain to the mitochondrial intermembrane spacelocalized, ubiquitous, and ancient Syk pathway in hematopoietic and nonhematopoietic cells.