A review of fluorescence methods for assessing labile iron in cells and biological fluids

Breno P. Espósito, Silvina Epsztejn, William Breuer, Z. Ioav Cabantchik*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

214 Scopus citations

Abstract

A variety of biochemical, pharmacological, and toxicological properties have been attributed to labile forms of iron that are associated with cells or with biological fluids. Unlike the major fraction of bioiron which is protein bound, the labile bioiron is chelatable and therefore amenable for detection by metal-sensing devices that are coupled to chelation moieties. The present review deals with the detection of various labile forms of iron present in living cells and in fluids of biological interest, in health and disease. The main focus of the review is on the design and application of fluorescent probes as analytical tools for assessing labile iron and iron transport mechanisms and the efficiency of iron chelators in solution and in cellular systems.

Original languageEnglish
Pages (from-to)1-18
Number of pages18
JournalAnalytical Biochemistry
Volume304
Issue number1
DOIs
StatePublished - 1 May 2002

Keywords

  • Chelators
  • Fluorescence
  • Iron
  • Oxidative stress
  • Transferrin
  • Transport

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