A robust method for critical micelle concentration determination using coumarin-6 as a fluorescent probe

Arnon Fluksman, Ofra Benny*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

56 Scopus citations


The critical micelle concentration (CMC) is the point at which micelles are self-assembled from surfactants added to the system. Determination of the CMC is commonly based on surface tension and conductivity measurements since these are relatively simple and well described. The dye micellization method performed using pyrene as a fluorescent probe, is limited like other methods by inaccuracy and the lack of a standard procedure. We established a simple, robust, and straightforward method based on dye micellization, using coumarin-6 as a fluorescent probe for CMC determination. We first optimized the method and demonstrated the problematic pyrene detection by a plate reader. We compared the novel method to the established surface tension method using anionic, cationic and non-ionic surfactants, and compared to reported values in the literature. We selected to measure sodium dodecyl sulfate using the conductivity method, followed by visualization by fluorescence microscopy. The values obtained by our method were reproducible and in accordance with the values reported in the literature, regardless of the ionic charge of the surfactant. Adopting such a robust protocol would be beneficial for pharmaceutical research and industry and has the potential to replace the more laborious and less accurate commonly available methods.

Original languageAmerican English
Pages (from-to)3810-3818
Number of pages9
JournalAnalytical Methods
Issue number30
StatePublished - 14 Aug 2019

Bibliographical note

Publisher Copyright:
© 2019 The Royal Society of Chemistry.


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