Abstract
A rapid, convenient, and highly sensitive fluorometric assay for α-chymotrypsin is suggested. The assay is based on the direct recording of the emission intensity of β-naphthol liberated by enzymic hydrolysis of ZPNE. Microquantities down to 5 × 10-2 ng of α-chymotrypsin are easily assayed. The advantage and applicability of this assay to biological preparations are discussed.
Original language | English |
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Pages (from-to) | 218-226 |
Number of pages | 9 |
Journal | Analytical Biochemistry |
Volume | 40 |
Issue number | 1 |
DOIs | |
State | Published - Mar 1971 |