A sensitive fluorometric assay for α-chymotrypsin

Elisha Haas; Yehudit Elkana; Richard G. Kulka

doi:10.1016/0003-2697(71)90095-9

A sensitive fluorometric assay for α-chymotrypsin

Elisha Haas^*
, Yehudit Elkana
, Richard G. Kulka

^*Corresponding author for this work

Alexander Silberman Institute of Life Sciences

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

A rapid, convenient, and highly sensitive fluorometric assay for α-chymotrypsin is suggested. The assay is based on the direct recording of the emission intensity of β-naphthol liberated by enzymic hydrolysis of ZPNE. Microquantities down to 5 × 10^-2 ng of α-chymotrypsin are easily assayed. The advantage and applicability of this assay to biological preparations are discussed.

Original language	English
Pages (from-to)	218-226
Number of pages	9
Journal	Analytical Biochemistry
Volume	40
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(71)90095-9
State	Published - Mar 1971

Access to Document

10.1016/0003-2697(71)90095-9

Cite this

@article{1d3debae320c46bf97f37e2dda2e36a8,

title = "A sensitive fluorometric assay for α-chymotrypsin",

abstract = "A rapid, convenient, and highly sensitive fluorometric assay for α-chymotrypsin is suggested. The assay is based on the direct recording of the emission intensity of β-naphthol liberated by enzymic hydrolysis of ZPNE. Microquantities down to 5 × 10-2 ng of α-chymotrypsin are easily assayed. The advantage and applicability of this assay to biological preparations are discussed.",

author = "Elisha Haas and Yehudit Elkana and Kulka, \{Richard G.\}",

year = "1971",

month = mar,

doi = "10.1016/0003-2697(71)90095-9",

language = "אנגלית",

volume = "40",

pages = "218--226",

journal = "Analytical Biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - A sensitive fluorometric assay for α-chymotrypsin

AU - Haas, Elisha

AU - Elkana, Yehudit

AU - Kulka, Richard G.

PY - 1971/3

Y1 - 1971/3

N2 - A rapid, convenient, and highly sensitive fluorometric assay for α-chymotrypsin is suggested. The assay is based on the direct recording of the emission intensity of β-naphthol liberated by enzymic hydrolysis of ZPNE. Microquantities down to 5 × 10-2 ng of α-chymotrypsin are easily assayed. The advantage and applicability of this assay to biological preparations are discussed.

AB - A rapid, convenient, and highly sensitive fluorometric assay for α-chymotrypsin is suggested. The assay is based on the direct recording of the emission intensity of β-naphthol liberated by enzymic hydrolysis of ZPNE. Microquantities down to 5 × 10-2 ng of α-chymotrypsin are easily assayed. The advantage and applicability of this assay to biological preparations are discussed.

UR - https://www.scopus.com/pages/publications/0015026689

U2 - 10.1016/0003-2697(71)90095-9

DO - 10.1016/0003-2697(71)90095-9

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

C2 - 5550147

AN - SCOPUS:0015026689

SN - 0003-2697

VL - 40

SP - 218

EP - 226

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 1

ER -

A sensitive fluorometric assay for α-chymotrypsin

Abstract

Access to Document

Other files and links

Fingerprint

Cite this