A Sensitive in vitro Method for Prolactin Determination

J. Mishkinsky; S. Dikstein; M. Ben-David; J. Azeroual; F. G. Sulman

doi:10.3181/00379727-125-32091

A Sensitive in vitro Method for Prolactin Determination

J. Mishkinsky
, S. Dikstein
, M. Ben-David
, J. Azeroual
, F. G. Sulman

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

The mid-part of the inguinal mammary gland of non-primed virgin, me-testric albino rats, weighing 270 ± 10 g, is sensitive to 0.001 I.U./ml of prolactin when cultivated in enriched synthetic medium M-199 for a period of 2 days. This method may replace the standard crop-gland method for prolactin assay and other routine tests for prolactin, because of its reproducibility, high accuracy and specificity. Its quantitative aspects are being worked out.

Original language	English
Pages (from-to)	360-363
Number of pages	4
Journal	Experimental Biology and Medicine
Volume	125
Issue number	2
DOIs	https://doi.org/10.3181/00379727-125-32091
State	Published - Jun 1967

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N2 - The mid-part of the inguinal mammary gland of non-primed virgin, me-testric albino rats, weighing 270 ± 10 g, is sensitive to 0.001 I.U./ml of prolactin when cultivated in enriched synthetic medium M-199 for a period of 2 days. This method may replace the standard crop-gland method for prolactin assay and other routine tests for prolactin, because of its reproducibility, high accuracy and specificity. Its quantitative aspects are being worked out.

AB - The mid-part of the inguinal mammary gland of non-primed virgin, me-testric albino rats, weighing 270 ± 10 g, is sensitive to 0.001 I.U./ml of prolactin when cultivated in enriched synthetic medium M-199 for a period of 2 days. This method may replace the standard crop-gland method for prolactin assay and other routine tests for prolactin, because of its reproducibility, high accuracy and specificity. Its quantitative aspects are being worked out.

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A Sensitive in vitro Method for Prolactin Determination

Abstract

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