A sensitive method for detecting cells coated with antibodies with human monoclonal rheumatoid factor produced in vitro

Michael Steinitz; Sara Tamir; Gad Spira; Wolfgang Siegert

doi:10.1016/0022-1759(82)90311-8

A sensitive method for detecting cells coated with antibodies with human monoclonal rheumatoid factor produced in vitro

Michael Steinitz^*, Sara Tamir, Gad Spira, Wolfgang Siegert

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

A human monoclonal rheumatoid factor (RF) produced in vitro by an Epstein-Barr virus immortalized cell line has been used to detect and quantitate antibodies specifically bound to cells. The RF is purified from cell culture supernatant by a simple procedure and then radiolabeled. In this assay, the binding of ¹²⁵I-labeled RF to cells coated with specific antibodies is determined. This RF method detects very low titers of antibodies directed against specific cellular antigens and also minorities of antibody coated tumor cells in mixtures with identical non-coated cells. The advantages of this unique human monoclonal antibody are discussed.

Original language	English
Pages (from-to)	273-280
Number of pages	8
Journal	Journal of Immunological Methods
Volume	54
Issue number	3
DOIs	https://doi.org/10.1016/0022-1759(82)90311-8
State	Published - 12 Nov 1982
Externally published	Yes

Keywords

EBV cell line
human monoclonal antibody
immune complexes
rheumatoid factor

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10.1016/0022-1759(82)90311-8

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title = "A sensitive method for detecting cells coated with antibodies with human monoclonal rheumatoid factor produced in vitro",

abstract = "A human monoclonal rheumatoid factor (RF) produced in vitro by an Epstein-Barr virus immortalized cell line has been used to detect and quantitate antibodies specifically bound to cells. The RF is purified from cell culture supernatant by a simple procedure and then radiolabeled. In this assay, the binding of 125I-labeled RF to cells coated with specific antibodies is determined. This RF method detects very low titers of antibodies directed against specific cellular antigens and also minorities of antibody coated tumor cells in mixtures with identical non-coated cells. The advantages of this unique human monoclonal antibody are discussed.",

keywords = "EBV cell line, human monoclonal antibody, immune complexes, rheumatoid factor",

author = "Michael Steinitz and Sara Tamir and Gad Spira and Wolfgang Siegert",

year = "1982",

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doi = "10.1016/0022-1759(82)90311-8",

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AU - Steinitz, Michael

AU - Tamir, Sara

AU - Spira, Gad

AU - Siegert, Wolfgang

PY - 1982/11/12

Y1 - 1982/11/12

N2 - A human monoclonal rheumatoid factor (RF) produced in vitro by an Epstein-Barr virus immortalized cell line has been used to detect and quantitate antibodies specifically bound to cells. The RF is purified from cell culture supernatant by a simple procedure and then radiolabeled. In this assay, the binding of 125I-labeled RF to cells coated with specific antibodies is determined. This RF method detects very low titers of antibodies directed against specific cellular antigens and also minorities of antibody coated tumor cells in mixtures with identical non-coated cells. The advantages of this unique human monoclonal antibody are discussed.

AB - A human monoclonal rheumatoid factor (RF) produced in vitro by an Epstein-Barr virus immortalized cell line has been used to detect and quantitate antibodies specifically bound to cells. The RF is purified from cell culture supernatant by a simple procedure and then radiolabeled. In this assay, the binding of 125I-labeled RF to cells coated with specific antibodies is determined. This RF method detects very low titers of antibodies directed against specific cellular antigens and also minorities of antibody coated tumor cells in mixtures with identical non-coated cells. The advantages of this unique human monoclonal antibody are discussed.

KW - EBV cell line

KW - human monoclonal antibody

KW - immune complexes

KW - rheumatoid factor

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EP - 280

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

IS - 3

ER -

A sensitive method for detecting cells coated with antibodies with human monoclonal rheumatoid factor produced in vitro

Abstract

Keywords

UN SDGs

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