TY - JOUR
T1 - A shared TCR CDR3 sequence in NOD mouse autoimmune diabetes
AU - Tikochinski, Yaron
AU - Elias, Dana
AU - Steeg, Christiana
AU - Marcus, Hadar
AU - Kantorowitz, Michael
AU - Reshef, Tamara
AU - Ablamunits, Vitaly
AU - Cohen, Irun R.
AU - Friedmann, Adam
PY - 1999
Y1 - 1999
N2 - T cells involved in autoimmune diseases have been characterized by the genetic elements used to construct their autoimmune TCR. In the present study, we sequenced the α and β chains of the TCR expressed by a CD4+ T cell clone, C9, functional in NOD mouse diabetes. Clone C9 can adoptively transfer diabetes or, when attenuated, C9 can be used to vaccinate NOD mice against diabetes. Clone C9 recognizes a peptide epitope (p277) of the 60 kDa heat shock protein (hsp60) molecule. We now report that the C9 TCR β chain features a CDR3 peptide sequence that is prevalent among NOD mice. This CDR3 element is detectable by 2 weeks of age in the thymus, and later in the spleen and in the autoimmune insulitis. Thus, a TCR CDR3β sequence appears to be a common idiotope associated with mouse diabetes.
AB - T cells involved in autoimmune diseases have been characterized by the genetic elements used to construct their autoimmune TCR. In the present study, we sequenced the α and β chains of the TCR expressed by a CD4+ T cell clone, C9, functional in NOD mouse diabetes. Clone C9 can adoptively transfer diabetes or, when attenuated, C9 can be used to vaccinate NOD mice against diabetes. Clone C9 recognizes a peptide epitope (p277) of the 60 kDa heat shock protein (hsp60) molecule. We now report that the C9 TCR β chain features a CDR3 peptide sequence that is prevalent among NOD mice. This CDR3 element is detectable by 2 weeks of age in the thymus, and later in the spleen and in the autoimmune insulitis. Thus, a TCR CDR3β sequence appears to be a common idiotope associated with mouse diabetes.
KW - CDR3
KW - Insulin-dependent diabetes mellitus
KW - TCR
UR - http://www.scopus.com/inward/record.url?scp=0033035367&partnerID=8YFLogxK
U2 - 10.1093/intimm/11.6.951
DO - 10.1093/intimm/11.6.951
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C2 - 10360969
AN - SCOPUS:0033035367
SN - 0953-8178
VL - 11
SP - 951
EP - 956
JO - International Immunology
JF - International Immunology
IS - 6
ER -