TY - JOUR
T1 - A shift in follicular fluid from triacylglycerols to membrane lipids is associated with positive pregnancy outcome
AU - Shehadeh, Alaa
AU - Bruck-Haimson, Reut
AU - Saidemberg, Daniel
AU - Zacharia, Anish
AU - Herzberg, Shmuel
AU - Ben-Meir, Assaf
AU - Moussaieff, Arieh
N1 - Publisher Copyright:
© FASEB
PY - 2019/9/1
Y1 - 2019/9/1
N2 - Follicular fluid (FF) is a liquid that surrounds the ovum. Its metabolite and, specifically, its lipid content have been associated with oocyte development. To characterize possible association between the lipid composition of FF and the outcome of pregnancy, we carried out a lipidomics study and compared the abundance of lipids from FF of patients with positive and negative outcomes. We found a differential lipid network wiring in positive-outcome FF, with a significant decrease (∼2 fold; P < 0.001) in triacylglycerol levels and higher accumulation (10–50%; P < 0.001) of membrane lipids groups (phospholipids and sphingolipids). In addition to this major metabolic alteration, other lipid groups such as cholesteryl esters showed lower levels in positive-outcome patients, whereas derivatives of vitamin D were highly accumulated in positive-outcome FF, supporting previous studies that associate vitamin D levels in FF to pregnancy outcome. Our data also point to specific lipid species with a differential accumulation pattern in positive-outcome FF that predicted pregnancy in a receiver operating characteristic analysis. Altogether, our results suggest that FF lipid network is associated with the oocyte development, with possible implications in diagnostics and treatment.—Shehadeh, A., Bruck-Haimson, R., Saidemberg, D., Zacharia. A., Herzberg, S., Ben-Meir, A., Moussaieff, A. A shift in follicular fluid from triacylglycerols to membrane lipids is associated with positive pregnancy outcome. FASEB J. 33, 10291–10299 (2019). www.fasebj.org.
AB - Follicular fluid (FF) is a liquid that surrounds the ovum. Its metabolite and, specifically, its lipid content have been associated with oocyte development. To characterize possible association between the lipid composition of FF and the outcome of pregnancy, we carried out a lipidomics study and compared the abundance of lipids from FF of patients with positive and negative outcomes. We found a differential lipid network wiring in positive-outcome FF, with a significant decrease (∼2 fold; P < 0.001) in triacylglycerol levels and higher accumulation (10–50%; P < 0.001) of membrane lipids groups (phospholipids and sphingolipids). In addition to this major metabolic alteration, other lipid groups such as cholesteryl esters showed lower levels in positive-outcome patients, whereas derivatives of vitamin D were highly accumulated in positive-outcome FF, supporting previous studies that associate vitamin D levels in FF to pregnancy outcome. Our data also point to specific lipid species with a differential accumulation pattern in positive-outcome FF that predicted pregnancy in a receiver operating characteristic analysis. Altogether, our results suggest that FF lipid network is associated with the oocyte development, with possible implications in diagnostics and treatment.—Shehadeh, A., Bruck-Haimson, R., Saidemberg, D., Zacharia. A., Herzberg, S., Ben-Meir, A., Moussaieff, A. A shift in follicular fluid from triacylglycerols to membrane lipids is associated with positive pregnancy outcome. FASEB J. 33, 10291–10299 (2019). www.fasebj.org.
KW - In vitro fertilization (IVF)
KW - lipidomics
KW - phospholipids
KW - sphingolipids
KW - vitamin D
UR - http://www.scopus.com/inward/record.url?scp=85071784739&partnerID=8YFLogxK
U2 - 10.1096/fj.201900318RR
DO - 10.1096/fj.201900318RR
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 31219705
AN - SCOPUS:85071784739
SN - 0892-6638
VL - 33
SP - 10291
EP - 10299
JO - FASEB Journal
JF - FASEB Journal
IS - 9
ER -