A shift in follicular fluid from triacylglycerols to membrane lipids is associated with positive pregnancy outcome

Alaa Shehadeh; Reut Bruck-Haimson; Daniel Saidemberg; Anish Zacharia; Shmuel Herzberg; Assaf Ben-Meir; Arieh Moussaieff

doi:10.1096/fj.201900318RR

A shift in follicular fluid from triacylglycerols to membrane lipids is associated with positive pregnancy outcome

Alaa Shehadeh, Reut Bruck-Haimson, Daniel Saidemberg, Anish Zacharia, Shmuel Herzberg, Assaf Ben-Meir, Arieh Moussaieff^*

^*Corresponding author for this work

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Follicular fluid (FF) is a liquid that surrounds the ovum. Its metabolite and, specifically, its lipid content have been associated with oocyte development. To characterize possible association between the lipid composition of FF and the outcome of pregnancy, we carried out a lipidomics study and compared the abundance of lipids from FF of patients with positive and negative outcomes. We found a differential lipid network wiring in positive-outcome FF, with a significant decrease (∼2 fold; P < 0.001) in triacylglycerol levels and higher accumulation (10–50%; P < 0.001) of membrane lipids groups (phospholipids and sphingolipids). In addition to this major metabolic alteration, other lipid groups such as cholesteryl esters showed lower levels in positive-outcome patients, whereas derivatives of vitamin D were highly accumulated in positive-outcome FF, supporting previous studies that associate vitamin D levels in FF to pregnancy outcome. Our data also point to specific lipid species with a differential accumulation pattern in positive-outcome FF that predicted pregnancy in a receiver operating characteristic analysis. Altogether, our results suggest that FF lipid network is associated with the oocyte development, with possible implications in diagnostics and treatment.—Shehadeh, A., Bruck-Haimson, R., Saidemberg, D., Zacharia. A., Herzberg, S., Ben-Meir, A., Moussaieff, A. A shift in follicular fluid from triacylglycerols to membrane lipids is associated with positive pregnancy outcome. FASEB J. 33, 10291–10299 (2019). www.fasebj.org.

Original language	American English
Pages (from-to)	10291-10299
Number of pages	9
Journal	FASEB Journal
Volume	33
Issue number	9
DOIs	https://doi.org/10.1096/fj.201900318RR
State	Published - 1 Sep 2019

Bibliographical note

Funding Information:
The authors thank Dr. Natalya Kogan (The Hebrew University of Jerusalem) for the kind help in preliminary experiments and method optimization and Dr. Yoav Smith (The Hebrew University of Jerusalem) for the kind help in performing the receiver operating characteristic analysis. The authors declare no conflicts of interest.

Publisher Copyright:
© FASEB

Keywords

In vitro fertilization (IVF)
lipidomics
phospholipids
sphingolipids
vitamin D

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10.1096/fj.201900318RR

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title = "A shift in follicular fluid from triacylglycerols to membrane lipids is associated with positive pregnancy outcome",

abstract = "Follicular fluid (FF) is a liquid that surrounds the ovum. Its metabolite and, specifically, its lipid content have been associated with oocyte development. To characterize possible association between the lipid composition of FF and the outcome of pregnancy, we carried out a lipidomics study and compared the abundance of lipids from FF of patients with positive and negative outcomes. We found a differential lipid network wiring in positive-outcome FF, with a significant decrease (∼2 fold; P < 0.001) in triacylglycerol levels and higher accumulation (10–50%; P < 0.001) of membrane lipids groups (phospholipids and sphingolipids). In addition to this major metabolic alteration, other lipid groups such as cholesteryl esters showed lower levels in positive-outcome patients, whereas derivatives of vitamin D were highly accumulated in positive-outcome FF, supporting previous studies that associate vitamin D levels in FF to pregnancy outcome. Our data also point to specific lipid species with a differential accumulation pattern in positive-outcome FF that predicted pregnancy in a receiver operating characteristic analysis. Altogether, our results suggest that FF lipid network is associated with the oocyte development, with possible implications in diagnostics and treatment.—Shehadeh, A., Bruck-Haimson, R., Saidemberg, D., Zacharia. A., Herzberg, S., Ben-Meir, A., Moussaieff, A. A shift in follicular fluid from triacylglycerols to membrane lipids is associated with positive pregnancy outcome. FASEB J. 33, 10291–10299 (2019). www.fasebj.org.",

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author = "Alaa Shehadeh and Reut Bruck-Haimson and Daniel Saidemberg and Anish Zacharia and Shmuel Herzberg and Assaf Ben-Meir and Arieh Moussaieff",

note = "Funding Information: The authors thank Dr. Natalya Kogan (The Hebrew University of Jerusalem) for the kind help in preliminary experiments and method optimization and Dr. Yoav Smith (The Hebrew University of Jerusalem) for the kind help in performing the receiver operating characteristic analysis. The authors declare no conflicts of interest. Publisher Copyright: {\textcopyright} FASEB",

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T1 - A shift in follicular fluid from triacylglycerols to membrane lipids is associated with positive pregnancy outcome

AU - Shehadeh, Alaa

AU - Bruck-Haimson, Reut

AU - Saidemberg, Daniel

AU - Zacharia, Anish

AU - Herzberg, Shmuel

AU - Ben-Meir, Assaf

AU - Moussaieff, Arieh

N1 - Funding Information: The authors thank Dr. Natalya Kogan (The Hebrew University of Jerusalem) for the kind help in preliminary experiments and method optimization and Dr. Yoav Smith (The Hebrew University of Jerusalem) for the kind help in performing the receiver operating characteristic analysis. The authors declare no conflicts of interest. Publisher Copyright: © FASEB

PY - 2019/9/1

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N2 - Follicular fluid (FF) is a liquid that surrounds the ovum. Its metabolite and, specifically, its lipid content have been associated with oocyte development. To characterize possible association between the lipid composition of FF and the outcome of pregnancy, we carried out a lipidomics study and compared the abundance of lipids from FF of patients with positive and negative outcomes. We found a differential lipid network wiring in positive-outcome FF, with a significant decrease (∼2 fold; P < 0.001) in triacylglycerol levels and higher accumulation (10–50%; P < 0.001) of membrane lipids groups (phospholipids and sphingolipids). In addition to this major metabolic alteration, other lipid groups such as cholesteryl esters showed lower levels in positive-outcome patients, whereas derivatives of vitamin D were highly accumulated in positive-outcome FF, supporting previous studies that associate vitamin D levels in FF to pregnancy outcome. Our data also point to specific lipid species with a differential accumulation pattern in positive-outcome FF that predicted pregnancy in a receiver operating characteristic analysis. Altogether, our results suggest that FF lipid network is associated with the oocyte development, with possible implications in diagnostics and treatment.—Shehadeh, A., Bruck-Haimson, R., Saidemberg, D., Zacharia. A., Herzberg, S., Ben-Meir, A., Moussaieff, A. A shift in follicular fluid from triacylglycerols to membrane lipids is associated with positive pregnancy outcome. FASEB J. 33, 10291–10299 (2019). www.fasebj.org.

AB - Follicular fluid (FF) is a liquid that surrounds the ovum. Its metabolite and, specifically, its lipid content have been associated with oocyte development. To characterize possible association between the lipid composition of FF and the outcome of pregnancy, we carried out a lipidomics study and compared the abundance of lipids from FF of patients with positive and negative outcomes. We found a differential lipid network wiring in positive-outcome FF, with a significant decrease (∼2 fold; P < 0.001) in triacylglycerol levels and higher accumulation (10–50%; P < 0.001) of membrane lipids groups (phospholipids and sphingolipids). In addition to this major metabolic alteration, other lipid groups such as cholesteryl esters showed lower levels in positive-outcome patients, whereas derivatives of vitamin D were highly accumulated in positive-outcome FF, supporting previous studies that associate vitamin D levels in FF to pregnancy outcome. Our data also point to specific lipid species with a differential accumulation pattern in positive-outcome FF that predicted pregnancy in a receiver operating characteristic analysis. Altogether, our results suggest that FF lipid network is associated with the oocyte development, with possible implications in diagnostics and treatment.—Shehadeh, A., Bruck-Haimson, R., Saidemberg, D., Zacharia. A., Herzberg, S., Ben-Meir, A., Moussaieff, A. A shift in follicular fluid from triacylglycerols to membrane lipids is associated with positive pregnancy outcome. FASEB J. 33, 10291–10299 (2019). www.fasebj.org.

KW - In vitro fertilization (IVF)

KW - lipidomics

KW - phospholipids

KW - sphingolipids

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DO - 10.1096/fj.201900318RR

M3 - Article

C2 - 31219705

AN - SCOPUS:85071784739

SN - 0892-6638

VL - 33

SP - 10291

EP - 10299

JO - FASEB Journal

JF - FASEB Journal

IS - 9

ER -

A shift in follicular fluid from triacylglycerols to membrane lipids is associated with positive pregnancy outcome

Abstract

Bibliographical note

Keywords

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