A simple method for identifying the palindromic sequences recognized by restriction endonucleases: the nucleotide sequence of the AvaII site

Camil Fuchs; Eric C. Rosenvold; Alik Honigman; Waclaw Szybalski

doi:10.1016/0378-1119(78)90011-2

A simple method for identifying the palindromic sequences recognized by restriction endonucleases: the nucleotide sequence of the AvaII site

Camil Fuchs, Eric C. Rosenvold, Alik Honigman, Waclaw Szybalski^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

Tables specifying the frequencies, distances between and positions of all possible tetra-, penta- and hexanucleotide palindromes in øX174 and SV40 viral DNAs were prepared by a computer search of their base sequences. A simple method based on these tables is described for identifying the sequence recognized by any specific restriction endonuclease. The method requires experimental determination of the number and approximate sizes of the fragments obtained by digestion of øX174 RF and SV40 DNAs. Using this method we identified the sequence for the AvaII restriction endonuclease as 5′-GG(_T^A)CC.

Original language	English
Pages (from-to)	1-23
Number of pages	23
Journal	Gene
Volume	4
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(78)90011-2
State	Published - Sep 1978
Externally published	Yes

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10.1016/0378-1119(78)90011-2

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title = "A simple method for identifying the palindromic sequences recognized by restriction endonucleases: the nucleotide sequence of the AvaII site",

abstract = "Tables specifying the frequencies, distances between and positions of all possible tetra-, penta- and hexanucleotide palindromes in {\o}X174 and SV40 viral DNAs were prepared by a computer search of their base sequences. A simple method based on these tables is described for identifying the sequence recognized by any specific restriction endonuclease. The method requires experimental determination of the number and approximate sizes of the fragments obtained by digestion of {\o}X174 RF and SV40 DNAs. Using this method we identified the sequence for the AvaII restriction endonuclease as 5′-GG(TA)CC.",

author = "Camil Fuchs and Rosenvold, {Eric C.} and Alik Honigman and Waclaw Szybalski",

year = "1978",

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doi = "10.1016/0378-1119(78)90011-2",

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AU - Szybalski, Waclaw

PY - 1978/9

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AB - Tables specifying the frequencies, distances between and positions of all possible tetra-, penta- and hexanucleotide palindromes in øX174 and SV40 viral DNAs were prepared by a computer search of their base sequences. A simple method based on these tables is described for identifying the sequence recognized by any specific restriction endonuclease. The method requires experimental determination of the number and approximate sizes of the fragments obtained by digestion of øX174 RF and SV40 DNAs. Using this method we identified the sequence for the AvaII restriction endonuclease as 5′-GG(TA)CC.

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A simple method for identifying the palindromic sequences recognized by restriction endonucleases: the nucleotide sequence of the AvaII site

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