A Simple Method for the Preparation of Homogeneous Phospholipid Vesicles

Y. Barenholz; D. Gibbes; B. J. Litman; J. Goll; T. E. Thompson; F. D. Carlson

doi:10.1021/bi00631a035

A Simple Method for the Preparation of Homogeneous Phospholipid Vesicles

Y. Barenholz, D. Gibbes, B. J. Litman, J. Goll^*, T. E. Thompson, F. D. Carlson

^*Corresponding author for this work

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Abstract

A new method is described for the preparation of homogeneously sized, single-lamellar phospholipid vesicles. This method, which is based on differential high-speed ultracentrifugation, has the advantages of a higher vesicle yield without dilution and rapidity of preparation when compared to the molecular-sieve technique. The homogeneity of vesicle dispersions, prepared by this new method, is examined by several physical techniques and found to be comparable to the best samples prepared by molecular-sieve chromatography.

Original language	English
Pages (from-to)	2806-2810
Number of pages	5
Journal	Biochemistry
Volume	16
Issue number	12
DOIs	https://doi.org/10.1021/bi00631a035
State	Published - 1 Jun 1977
Externally published	Yes

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10.1021/bi00631a035

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abstract = "A new method is described for the preparation of homogeneously sized, single-lamellar phospholipid vesicles. This method, which is based on differential high-speed ultracentrifugation, has the advantages of a higher vesicle yield without dilution and rapidity of preparation when compared to the molecular-sieve technique. The homogeneity of vesicle dispersions, prepared by this new method, is examined by several physical techniques and found to be comparable to the best samples prepared by molecular-sieve chromatography.",

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AU - Barenholz, Y.

AU - Gibbes, D.

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AU - Thompson, T. E.

AU - Carlson, F. D.

PY - 1977/6/1

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N2 - A new method is described for the preparation of homogeneously sized, single-lamellar phospholipid vesicles. This method, which is based on differential high-speed ultracentrifugation, has the advantages of a higher vesicle yield without dilution and rapidity of preparation when compared to the molecular-sieve technique. The homogeneity of vesicle dispersions, prepared by this new method, is examined by several physical techniques and found to be comparable to the best samples prepared by molecular-sieve chromatography.

AB - A new method is described for the preparation of homogeneously sized, single-lamellar phospholipid vesicles. This method, which is based on differential high-speed ultracentrifugation, has the advantages of a higher vesicle yield without dilution and rapidity of preparation when compared to the molecular-sieve technique. The homogeneity of vesicle dispersions, prepared by this new method, is examined by several physical techniques and found to be comparable to the best samples prepared by molecular-sieve chromatography.

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A Simple Method for the Preparation of Homogeneous Phospholipid Vesicles

Abstract

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