A Simple Method for the Preparation of Homogeneous Phospholipid Vesicles

A new method is described for the preparation of homogeneously sized, single-lamellar phospholipid vesicles. This method, which is based on differential high-speed ultracentrifugation, has the advantages of a higher vesicle yield without dilution and rapidity of preparation when compared to the molecular-sieve technique. The homogeneity of vesicle dispersions, prepared by this new method, is examined by several physical techniques and found to be comparable to the best samples prepared by molecular-sieve chromatography.