TY - JOUR
T1 - A simple plate-assay for the screening of l-malic acid producing microorganisms
AU - Peleg, Y.
AU - Rokem, J. S.
AU - Goldberg, I.
PY - 1990/2
Y1 - 1990/2
N2 - A simple plate-assay has been developed to screen microorganisms for l-malic acid production. Acid producing organisms were identified, after microbial colony growth on media containing glucose or fumaric acid as sole carbon sources, by formation of a dark halo of formazan. The halo was observed when the plate was covered with a soft agar overlay containing NAD+-malate dehydrogenase, NAD+, phenazine methosulfate (PMS) and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetralzolium bromide (MTT). The assay developed is simple, specific for l-malic acid and therefore can be used to identify l-malic acid producing filamentous fungi using glucose as carbon source (e.g. Aspergillus strain). The assay is also applicable for screening bacteria with high fumarase activity, able to convert fumaric acid to l-malic acid.
AB - A simple plate-assay has been developed to screen microorganisms for l-malic acid production. Acid producing organisms were identified, after microbial colony growth on media containing glucose or fumaric acid as sole carbon sources, by formation of a dark halo of formazan. The halo was observed when the plate was covered with a soft agar overlay containing NAD+-malate dehydrogenase, NAD+, phenazine methosulfate (PMS) and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetralzolium bromide (MTT). The assay developed is simple, specific for l-malic acid and therefore can be used to identify l-malic acid producing filamentous fungi using glucose as carbon source (e.g. Aspergillus strain). The assay is also applicable for screening bacteria with high fumarase activity, able to convert fumaric acid to l-malic acid.
KW - Plate-assay
KW - l-Malic acid
UR - http://www.scopus.com/inward/record.url?scp=0025190013&partnerID=8YFLogxK
U2 - 10.1016/0378-1097(90)90001-7
DO - 10.1016/0378-1097(90)90001-7
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C2 - 2182383
AN - SCOPUS:0025190013
SN - 0378-1097
VL - 67
SP - 233
EP - 236
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 3
ER -