A simplified and efficient method for single-step, targeted gene deletion in mycobacteria

Yahav Bracha, Daniel Barkan*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Targeted gene deletion in mycobacteria remain complicated, requiring expertise and multiple steps. Here we present a single-step, easy to understand and perform method for targeted gene deletion. Using this method, we successfully deleted several genes in both M. smegmatis and M. abscessus. We believe this method will facilitate molecular research of mycobacteria and make it accessible to a greater number of researchers throughout the world.

Original languageAmerican English
Article number102520
JournalTuberculosis
Volume147
DOIs
StatePublished - Jul 2024

Bibliographical note

Publisher Copyright:
© 2024 Elsevier Ltd

Keywords

  • Gene deletion
  • Genetic manipulation
  • Mycobacteria

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