A simplified and efficient method for single-step, targeted gene deletion in mycobacteria

Yahav Bracha; Daniel Barkan

doi:10.1016/j.tube.2024.102520

A simplified and efficient method for single-step, targeted gene deletion in mycobacteria

Yahav Bracha, Daniel Barkan^*

^*Corresponding author for this work

The Koret School of Veterinary Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Targeted gene deletion in mycobacteria remain complicated, requiring expertise and multiple steps. Here we present a single-step, easy to understand and perform method for targeted gene deletion. Using this method, we successfully deleted several genes in both M. smegmatis and M. abscessus. We believe this method will facilitate molecular research of mycobacteria and make it accessible to a greater number of researchers throughout the world.

Original language	English
Article number	102520
Journal	Tuberculosis
Volume	147
DOIs	https://doi.org/10.1016/j.tube.2024.102520
State	Published - Jul 2024

Bibliographical note

Publisher Copyright:
© 2024 Elsevier Ltd

Keywords

Gene deletion
Genetic manipulation
Mycobacteria

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10.1016/j.tube.2024.102520

Cite this

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abstract = "Targeted gene deletion in mycobacteria remain complicated, requiring expertise and multiple steps. Here we present a single-step, easy to understand and perform method for targeted gene deletion. Using this method, we successfully deleted several genes in both M. smegmatis and M. abscessus. We believe this method will facilitate molecular research of mycobacteria and make it accessible to a greater number of researchers throughout the world.",

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AB - Targeted gene deletion in mycobacteria remain complicated, requiring expertise and multiple steps. Here we present a single-step, easy to understand and perform method for targeted gene deletion. Using this method, we successfully deleted several genes in both M. smegmatis and M. abscessus. We believe this method will facilitate molecular research of mycobacteria and make it accessible to a greater number of researchers throughout the world.

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KW - Genetic manipulation

KW - Mycobacteria

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A simplified and efficient method for single-step, targeted gene deletion in mycobacteria

Abstract

Bibliographical note

Keywords

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