TY - JOUR
T1 - A spectrophotometric method for determination of sphingomyelinase
AU - Gatt, S.
AU - Dinur, T.
AU - Barenholz, Y.
PY - 1978/9/28
Y1 - 1978/9/28
N2 - A colored derivative of sphingomyelin was synthesized and used as substrate for several sphingomyelinases. The compound is N-ω-trinitrophenyl aminolaurylsphingosylphosphorylcholine. The rate of hydrolysis of this substrate was compared to that of bovine brain sphingomyelin, labelled with tritium in the choline moiety. The following enzyme preparations were used: homogenate-less debris of brain, assayed at pH 5.0 or 7.4; a solubilized preparation derived from rat brain lysosomes, assayed at pH 5.0 and a purified enzyme of Staphylococcus aureus. With all preparations, the rates of hydrolysis of the yellow derivative were very similar to those of the brain sphingomyelin. Extracts of skin fibroblasts of normal and Niemann-Pick patients as well as amniotic cells were also used. Again, the rates of hydrolysis of the yellow derivative practically equalled those using brain sphingomyelin.
AB - A colored derivative of sphingomyelin was synthesized and used as substrate for several sphingomyelinases. The compound is N-ω-trinitrophenyl aminolaurylsphingosylphosphorylcholine. The rate of hydrolysis of this substrate was compared to that of bovine brain sphingomyelin, labelled with tritium in the choline moiety. The following enzyme preparations were used: homogenate-less debris of brain, assayed at pH 5.0 or 7.4; a solubilized preparation derived from rat brain lysosomes, assayed at pH 5.0 and a purified enzyme of Staphylococcus aureus. With all preparations, the rates of hydrolysis of the yellow derivative were very similar to those of the brain sphingomyelin. Extracts of skin fibroblasts of normal and Niemann-Pick patients as well as amniotic cells were also used. Again, the rates of hydrolysis of the yellow derivative practically equalled those using brain sphingomyelin.
UR - http://www.scopus.com/inward/record.url?scp=0018180520&partnerID=8YFLogxK
U2 - 10.1016/0005-2760(78)90169-8
DO - 10.1016/0005-2760(78)90169-8
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 212111
AN - SCOPUS:0018180520
SN - 0005-2760
VL - 530
SP - 503
EP - 507
JO - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
JF - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
IS - 3
ER -