A temperature-sensitive mutation in asparaginyl-tRNA synthetase causes cell-cycle arrest in early S phase

Gill Diamond, Howard Cedar*, Menashe Marcus

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

The Chinese hamster temperature-sensitive cell-cycle mutant ts24 was analyzed biochemicallly in order to determine the nature of this lesion. The inability of these cells to proceed through S phase at the restrictive temperature could be complemented by the addition of asparagine to the growth medium, and enzymological analysis showed that this line contains a temperature-sensitive asparaginyl-tRNA synthetase. Normal asparaginyl-tRNA synthetase activity was restored in cells transfected with cloned genomic DNA that overcomes the mutational defect. In corroboration with these results it was shown that a different temperature-sensitive asparaginyl-tRNA synthetase mutant isolated in another laboratory was blocked in S phase in a manner similar to that of ts24. While the mechanism by which asparaginyl-tRNA synthetase affects cell-cycle progression has not been elucidated, it can be shown that it is not mediated through alteration in overall levels of protein synthesis.

Original languageEnglish
Pages (from-to)53-60
Number of pages8
JournalExperimental Cell Research
Volume184
Issue number1
DOIs
StatePublished - Sep 1989

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