Abstract
The Chinese hamster temperature-sensitive cell-cycle mutant ts24 was analyzed biochemicallly in order to determine the nature of this lesion. The inability of these cells to proceed through S phase at the restrictive temperature could be complemented by the addition of asparagine to the growth medium, and enzymological analysis showed that this line contains a temperature-sensitive asparaginyl-tRNA synthetase. Normal asparaginyl-tRNA synthetase activity was restored in cells transfected with cloned genomic DNA that overcomes the mutational defect. In corroboration with these results it was shown that a different temperature-sensitive asparaginyl-tRNA synthetase mutant isolated in another laboratory was blocked in S phase in a manner similar to that of ts24. While the mechanism by which asparaginyl-tRNA synthetase affects cell-cycle progression has not been elucidated, it can be shown that it is not mediated through alteration in overall levels of protein synthesis.
Original language | English |
---|---|
Pages (from-to) | 53-60 |
Number of pages | 8 |
Journal | Experimental Cell Research |
Volume | 184 |
Issue number | 1 |
DOIs | |
State | Published - Sep 1989 |