TY - JOUR
T1 - A theoretical experimental method to determine the locus of desensitization
AU - Buchman, E.
AU - Parnas, H.
PY - 1999/9
Y1 - 1999/9
N2 - Consider a ligand-gated channel with n agonist binding sites which can undergo desensitization. We present a theoretical experimental procedure for pinpointing the principal receptor state from which there is a transition to the desensitized state. The method is based on the observation that the dependence of the slope of the time constant of desensitization vs agonist concentration, at low concentrations, represents the state from which desensitization occurs. In those receptors where desensitization occurs from the open state (or the one immediately preceding it), the method also enables us to determine the number of binding sites.
AB - Consider a ligand-gated channel with n agonist binding sites which can undergo desensitization. We present a theoretical experimental procedure for pinpointing the principal receptor state from which there is a transition to the desensitized state. The method is based on the observation that the dependence of the slope of the time constant of desensitization vs agonist concentration, at low concentrations, represents the state from which desensitization occurs. In those receptors where desensitization occurs from the open state (or the one immediately preceding it), the method also enables us to determine the number of binding sites.
UR - http://www.scopus.com/inward/record.url?scp=0032852296&partnerID=8YFLogxK
U2 - 10.1006/bulm.1999.0121
DO - 10.1006/bulm.1999.0121
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C2 - 17886752
AN - SCOPUS:0032852296
SN - 0092-8240
VL - 61
SP - 963
EP - 986
JO - Bulletin of Mathematical Biology
JF - Bulletin of Mathematical Biology
IS - 5
ER -