Abstract
Halobacterium sodomense, a halophilic bacterium from the Dead Sea, degraded starch to glucose by means of an extracellular amyloglucosidase with a temperature optimum of around 65°C in the presence of 1.4 M NaCl, and around 75°C in the presence of 3.9 M NaCl. The enzyme required salt concentrations higher than 1 M for optimal activity, NaCl, KCl, and MgCl2 being equally suitable as activators. The optimum pH was 7.5. H. sodomense culture supernatants showed only a very low maltose degrading activity. H. sodomense excreted amyloglucosidase constitutively, and relatively high activities were found in cultures grown in the absence of starch; when glucose was added to the growth medium, the amount of enzyme excreted into the medium decreased.
Original language | American English |
---|---|
Pages (from-to) | 225-230 |
Number of pages | 6 |
Journal | Current Microbiology |
Volume | 8 |
Issue number | 4 |
DOIs | |
State | Published - Jul 1983 |
Externally published | Yes |