A 'tool box' for deciphering neuronal circuits in the developing chick spinal cord

Yoav Hadas; Alex Etlin; Haya Falk; Oshri Avraham; Oren Kobiler; Amos Panet; Aharon Lev-Tov; Avihu Klar

doi:10.1093/nar/gku750

A 'tool box' for deciphering neuronal circuits in the developing chick spinal cord

Yoav Hadas, Alex Etlin, Haya Falk, Oshri Avraham, Oren Kobiler, Amos Panet, Aharon Lev-Tov, Avihu Klar^*

^*Corresponding author for this work

Department of Medical Neurobiology

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

The genetic dissection of spinal circuits is an essential new means for understanding the neural basis of mammalian behavior. Molecular targeting of specific neuronal populations, a key instrument in the genetic dissection of neuronal circuits in the mouse model, is a complex and time-demanding process. Here we present a circuit-deciphering 'tool box' for fast, reliable and cheap genetic targeting of neuronal circuits in the developing spinal cord of the chick.We demonstrate targeting of motoneurons and spinal interneurons, mapping of axonal trajectories and synaptic targeting in both single and populations of spinal interneurons, and viral vector-mediated labeling of pre-motoneurons. We also demonstrate fluorescent imaging of the activity pattern of defined spinal neurons during rhythmicmotor behavior, and assess the role of channel rhodopsin-targeted population of interneurons in rhythmic behavior using specific photoactivation.

Original language	American English
Article number	e148
Journal	Nucleic Acids Research
Volume	42
Issue number	19
DOIs	https://doi.org/10.1093/nar/gku750
State	Published - 29 Oct 2014

Bibliographical note

Funding Information:
The authors thank Atrur Kania, Michael O''Donovan and Marysia Placzek for their helpful comments on the manuscript.We also express thanks to Omer Gal and Hiba Shihadeh for the technical assistance they provided. Legacy Heritage Biomedical Science Partnership [1930/08 to A.K and A.L-T]; and Israel Science Foundation [631/13 to A.K; 1591/08 and 491/12 to A.L-T]. Funding for open access charge: Legacy Heritage Biomedical Science Partnership [1930/08 to A.K and A.L-T]; and Israel Science Foundation [631/13 to A.K; 1591/08 and 491/12 to A.L-T]. Conflict of interest statement. None declared.

Publisher Copyright:
© The Author(s) 2014.

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title = "A 'tool box' for deciphering neuronal circuits in the developing chick spinal cord",

abstract = "The genetic dissection of spinal circuits is an essential new means for understanding the neural basis of mammalian behavior. Molecular targeting of specific neuronal populations, a key instrument in the genetic dissection of neuronal circuits in the mouse model, is a complex and time-demanding process. Here we present a circuit-deciphering 'tool box' for fast, reliable and cheap genetic targeting of neuronal circuits in the developing spinal cord of the chick.We demonstrate targeting of motoneurons and spinal interneurons, mapping of axonal trajectories and synaptic targeting in both single and populations of spinal interneurons, and viral vector-mediated labeling of pre-motoneurons. We also demonstrate fluorescent imaging of the activity pattern of defined spinal neurons during rhythmicmotor behavior, and assess the role of channel rhodopsin-targeted population of interneurons in rhythmic behavior using specific photoactivation.",

author = "Yoav Hadas and Alex Etlin and Haya Falk and Oshri Avraham and Oren Kobiler and Amos Panet and Aharon Lev-Tov and Avihu Klar",

note = "Funding Information: The authors thank Atrur Kania, Michael O''Donovan and Marysia Placzek for their helpful comments on the manuscript.We also express thanks to Omer Gal and Hiba Shihadeh for the technical assistance they provided. Legacy Heritage Biomedical Science Partnership [1930/08 to A.K and A.L-T]; and Israel Science Foundation [631/13 to A.K; 1591/08 and 491/12 to A.L-T]. Funding for open access charge: Legacy Heritage Biomedical Science Partnership [1930/08 to A.K and A.L-T]; and Israel Science Foundation [631/13 to A.K; 1591/08 and 491/12 to A.L-T]. Conflict of interest statement. None declared. Publisher Copyright: {\textcopyright} The Author(s) 2014.",

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A 'tool box' for deciphering neuronal circuits in the developing chick spinal cord

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