A two-step model for the localization of maternal mRNA in Xenopus oocytes: Involvement of microtubules and microfilaments in the translocation and anchoring of Vg1 mRNA

Joel K. Yisraeli*, Sergei Sokol, D. A. Melton

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

384 Scopus citations

Abstract

In an effort to understand how polarity is established in Xenopus oocytes, we have analyzed the process of localization of the maternal mRNA, Vg1. In fully grown oocytes, Vg1 mRNA is tightly localized at the vegetal cortex. Biochemical fractionation shows that the mRNA is preferentially associated with a detergent-insoluble subcellular fraction. The use of cytoskeletal inhibitors suggests that (1) microtubules are involved in the translocation of the message to the vegetal hemisphere and (2) microfilaments are important for the anchoring of the message at the cortex. Furthermore, immunohistochemistry reveals that a cytoplasmic microtubule array exists during translocation. These results suggest a role for the cytoskeleton in localizing information in the oocyte.

Original languageAmerican English
Pages (from-to)289-298
Number of pages10
JournalJournal of Embryology and Experimental Morphology
Volume108
Issue number2
StatePublished - Feb 1990
Externally publishedYes

Keywords

  • Localization
  • Maternal mRNA
  • Micro tubules
  • Microfilaments
  • Polarity
  • Vg1
  • Xenopus oocytes

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