TY - JOUR
T1 - A unique ATP-dependent DNA topoisomerase from trypanosomatids.
AU - Shlomai, J.
AU - Zadok, A.
AU - Frank, D.
PY - 1984
Y1 - 1984
N2 - Crithidia fasciculata DNA topoisomerase (22) has been purified to near homogeneity from trypanosomatid cell extracts. The purified enzyme catalyzes the reversible interconversion of monomeric duplex DNA circles and catenanes in an ATP dependent reaction. Reversible catenane formation is affected by the ionic strength and is dependent upon the action of a crithidial DNA binding protein, which could be substituted for the polyamine spermidine. Covalently sealed DNA circles are specifically used as substrates for decatenation. Nicking, but not relaxation per se, inhibits network decatenation and has little or no effect on catenane formation. The catalytic properties of this enzyme and its potential role in the prereplication release and post replication reattachment of kDNA minicircles are discussed.
AB - Crithidia fasciculata DNA topoisomerase (22) has been purified to near homogeneity from trypanosomatid cell extracts. The purified enzyme catalyzes the reversible interconversion of monomeric duplex DNA circles and catenanes in an ATP dependent reaction. Reversible catenane formation is affected by the ionic strength and is dependent upon the action of a crithidial DNA binding protein, which could be substituted for the polyamine spermidine. Covalently sealed DNA circles are specifically used as substrates for decatenation. Nicking, but not relaxation per se, inhibits network decatenation and has little or no effect on catenane formation. The catalytic properties of this enzyme and its potential role in the prereplication release and post replication reattachment of kDNA minicircles are discussed.
UR - http://www.scopus.com/inward/record.url?scp=0021669696&partnerID=8YFLogxK
U2 - 10.1007/978-1-4684-8730-5_42
DO - 10.1007/978-1-4684-8730-5_42
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C2 - 6098160
AN - SCOPUS:0021669696
SN - 0065-2598
VL - 179
SP - 409
EP - 422
JO - Advances in Experimental Medicine and Biology
JF - Advances in Experimental Medicine and Biology
ER -