A Vibrational Analysis of Rhodopsin and Bacteriorhodopsin Chromophore Analogues: Resonance Raman and Infrared Spectroscopy of Chemically Modified Retinals and Schiff Bases

Aaron Lewis, Ann T. Lemley, Robert E. Cookingham

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41 Scopus citations

Abstract

Resonance Raman spectroscopy has been used to study chemically modified retinal analogues involving chain substitutions, ring substitutions, or Schiff-base linkages. In addition, retinal fragments and fully deuterated retinals were investigated, and infrared spectra of the four isomers of retinal were obtained. Low-frequency resonance Raman spectra are also reported for all of the isomers of retinal, for the protonated and unprotonated Schiff bases of frans-retinal, for β-ionone, and for trans-3-dehydroretinal. Band assignments were made to specific vibrational motions, and these assignments have led to a detailed understanding of the spectral features observed in the resonance Raman spectra of the retinylidene chromophore in rhodopsin and bacteriorhodopsin.

Original languageEnglish
Pages (from-to)4699-4711
Number of pages13
JournalBiochemistry
Volume17
Issue number22
DOIs
StatePublished - 1978
Externally publishedYes

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