A zinc selective oxytocin based biosensor

Evgeniy Mervinetsky; Israel Alshanski; Kiran Kumar Tadi; Mattan Hurevich; Shlomo Yitzchaik; Arezoo Dianat; Jörg Buchwald; Rafael Gutierrez; Gianaurelio Cuniberti

doi:10.1039/c9tb01932d

A zinc selective oxytocin based biosensor

Evgeniy Mervinetsky, Israel Alshanski, Kiran Kumar Tadi, Mattan Hurevich, Shlomo Yitzchaik, Arezoo Dianat, Jörg Buchwald, Rafael Gutierrez^*, Gianaurelio Cuniberti

^*Corresponding author for this work

Institute of Chemistry

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Oxytocin is a peptide hormone with high affinity to both Zn²⁺ and Cu²⁺ ions compared to other metal ions. This affinity makes oxytocin an attractive recognition layer for monitoring the levels of these essential ions in biofluids. Native oxytocin cannot differentiate between Cu²⁺ and Zn²⁺ ions and hence it is not useful for sensing Zn²⁺ in the presence of Cu²⁺. We elucidated the effect of the terminal amine group of oxytocin on the affinity toward Cu²⁺ using theoretical calculations. We designed a new Zn²⁺ selective oxytocin-based biosensor that utilizes the terminal amine for surface anchoring, also preventing the response to Cu²⁺. The biosensor shows exceptional selectivity and very high sensitivity to Zn²⁺ in impedimetric biosensing.

Original language	English
Pages (from-to)	155-160
Number of pages	6
Journal	Journal of Materials Chemistry B
Volume	8
Issue number	1
DOIs	https://doi.org/10.1039/c9tb01932d
State	Published - 1 Jan 2020

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This journal is © The Royal Society of Chemistry.

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abstract = "Oxytocin is a peptide hormone with high affinity to both Zn2+ and Cu2+ ions compared to other metal ions. This affinity makes oxytocin an attractive recognition layer for monitoring the levels of these essential ions in biofluids. Native oxytocin cannot differentiate between Cu2+ and Zn2+ ions and hence it is not useful for sensing Zn2+ in the presence of Cu2+. We elucidated the effect of the terminal amine group of oxytocin on the affinity toward Cu2+ using theoretical calculations. We designed a new Zn2+ selective oxytocin-based biosensor that utilizes the terminal amine for surface anchoring, also preventing the response to Cu2+. The biosensor shows exceptional selectivity and very high sensitivity to Zn2+ in impedimetric biosensing.",

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AU - Mervinetsky, Evgeniy

AU - Alshanski, Israel

AU - Tadi, Kiran Kumar

AU - Hurevich, Mattan

AU - Yitzchaik, Shlomo

AU - Dianat, Arezoo

AU - Buchwald, Jörg

AU - Gutierrez, Rafael

AU - Cuniberti, Gianaurelio

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A zinc selective oxytocin based biosensor

Abstract

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