Aberrant inflammation and resistance to glucocorticoids in annexin 1-/- mouse.

Robert Hannon; Jamie D. Croxtall; Steve J. Getting; Fiorita Roviezzo; Simon Yona; Mark J. Paul-Clark; Felicity N.E. Gavins; Mauro Perretti; John F. Morris; Julia C. Buckingham; Roderick J. Flower

doi:10.1096/fj.02-0239fje

Aberrant inflammation and resistance to glucocorticoids in annexin 1-/- mouse.

Robert Hannon^*, Jamie D. Croxtall, Steve J. Getting, Fiorita Roviezzo, Simon Yona, Mark J. Paul-Clark, Felicity N.E. Gavins, Mauro Perretti, John F. Morris, Julia C. Buckingham, Roderick J. Flower

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

313 Scopus citations

Abstract

The 37-kDa protein annexin 1 (Anx-1; lipocortin 1) has been implicated in the regulation of phagocytosis, cell signaling, and proliferation and is postulated to be a mediator of glucocorticoid action in inflammation and in the control of anterior pituitary hormone release. Here, we report that mice lacking the Anx-1 gene exhibit a complex phenotype that includes an altered expression of other annexins as well as of COX-2 and cPLA2. In carrageenin- or zymosan-induced inflammation, Anx-1-/- mice exhibit an exaggerated response to the stimuli characterized by an increase in leukocyte emigration and IL-1beta generation and a partial or complete resistance to the antiinflammatory effects of glucocorticoids. Anx-1-/- polymorphonuclear leucocytes exhibited increased spontaneous migratory behavior in vivo whereas in vitro, leukocytes from Anx-1-/- mice had reduced cell surface CD 11b (MAC-1) but enhanced CD62L (L-selectin) expression and Anx-1-/- macrophages exhibited anomalies in phagocytosis. There are also gender differences in activated leukocyte behavior in the Anx-1-/- mice that are not seen in the wild-type animals, suggesting an interaction between sex hormones and inflammation in Anx-1-/- animals.

Original language	English
Pages (from-to)	253-255
Number of pages	3
Journal	FASEB Journal
Volume	17
Issue number	2
DOIs	https://doi.org/10.1096/fj.02-0239fje
State	Published - Feb 2003
Externally published	Yes

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title = "Aberrant inflammation and resistance to glucocorticoids in annexin 1-/- mouse.",

abstract = "The 37-kDa protein annexin 1 (Anx-1; lipocortin 1) has been implicated in the regulation of phagocytosis, cell signaling, and proliferation and is postulated to be a mediator of glucocorticoid action in inflammation and in the control of anterior pituitary hormone release. Here, we report that mice lacking the Anx-1 gene exhibit a complex phenotype that includes an altered expression of other annexins as well as of COX-2 and cPLA2. In carrageenin- or zymosan-induced inflammation, Anx-1-/- mice exhibit an exaggerated response to the stimuli characterized by an increase in leukocyte emigration and IL-1beta generation and a partial or complete resistance to the antiinflammatory effects of glucocorticoids. Anx-1-/- polymorphonuclear leucocytes exhibited increased spontaneous migratory behavior in vivo whereas in vitro, leukocytes from Anx-1-/- mice had reduced cell surface CD 11b (MAC-1) but enhanced CD62L (L-selectin) expression and Anx-1-/- macrophages exhibited anomalies in phagocytosis. There are also gender differences in activated leukocyte behavior in the Anx-1-/- mice that are not seen in the wild-type animals, suggesting an interaction between sex hormones and inflammation in Anx-1-/- animals.",

author = "Robert Hannon and Croxtall, {Jamie D.} and Getting, {Steve J.} and Fiorita Roviezzo and Simon Yona and Paul-Clark, {Mark J.} and Gavins, {Felicity N.E.} and Mauro Perretti and Morris, {John F.} and Buckingham, {Julia C.} and Flower, {Roderick J.}",

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AU - Croxtall, Jamie D.

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AU - Roviezzo, Fiorita

AU - Yona, Simon

AU - Paul-Clark, Mark J.

AU - Gavins, Felicity N.E.

AU - Perretti, Mauro

AU - Morris, John F.

AU - Buckingham, Julia C.

AU - Flower, Roderick J.

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AB - The 37-kDa protein annexin 1 (Anx-1; lipocortin 1) has been implicated in the regulation of phagocytosis, cell signaling, and proliferation and is postulated to be a mediator of glucocorticoid action in inflammation and in the control of anterior pituitary hormone release. Here, we report that mice lacking the Anx-1 gene exhibit a complex phenotype that includes an altered expression of other annexins as well as of COX-2 and cPLA2. In carrageenin- or zymosan-induced inflammation, Anx-1-/- mice exhibit an exaggerated response to the stimuli characterized by an increase in leukocyte emigration and IL-1beta generation and a partial or complete resistance to the antiinflammatory effects of glucocorticoids. Anx-1-/- polymorphonuclear leucocytes exhibited increased spontaneous migratory behavior in vivo whereas in vitro, leukocytes from Anx-1-/- mice had reduced cell surface CD 11b (MAC-1) but enhanced CD62L (L-selectin) expression and Anx-1-/- macrophages exhibited anomalies in phagocytosis. There are also gender differences in activated leukocyte behavior in the Anx-1-/- mice that are not seen in the wild-type animals, suggesting an interaction between sex hormones and inflammation in Anx-1-/- animals.

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Aberrant inflammation and resistance to glucocorticoids in annexin 1-/- mouse.

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