Abortive Complexes of α-Amylases with Lanthanides

Alexander Levitzki; Jacques Reuben

doi:10.1021/bi00725a007

Abortive Complexes of α-Amylases with Lanthanides

Alexander Levitzki^*, Jacques Reuben

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Apoamylases from both hog pancreas and Bacillus subtilis were prepared by removing the tightly bound Ca²⁺. In both cases lanthanide ions fail to activate the enzyme. Furthermore, in both cases the rare earth ions do not compete for the essential Ca²⁺ binding site and therefore do not inhibit the Ca²⁺ activation process. B. subtilis α-amylase possesses two Ca²⁺ binding sites, one of which can bind Gd³⁺, with an additional site for the lanthanide ion.

Original language	English
Pages (from-to)	41-44
Number of pages	4
Journal	Biochemistry
Volume	12
Issue number	1
DOIs	https://doi.org/10.1021/bi00725a007
State	Published - 1 Jan 1973
Externally published	Yes

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Abortive Complexes of α-Amylases with Lanthanides

Abstract

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