TY - JOUR
T1 - Absence of major histocompatibility complex restriction in the interaction between processed basic protein and Ia of two rat strains
AU - Friedman, A.
AU - Lider, O.
AU - Beraud, E.
AU - Cohen, I. R.
PY - 1989
Y1 - 1989
N2 - Antigen fragments, biologically degraded by antigen-presenting cells (APC), combine with Ia positive moieties (IPM) to stimulate antigen-specific T lymphocyte lines. The main objective of this study was to evaluate whether this interaction was determined by the major histocompatibility complex (MHC) genotype of the APC, thus genetically restricting antigen-specific T lymphocyte proliferative responses. To do so, we assayed the capacity of processed basic protein, associated with IPM, to stimulate basic protein specific T lymphocyte lines derived from the Lewis (LW), Brown Norway (BN), and (LW x BN)F1 rat strains. Our findings are that: (a) IPM replaced the requirement for intact APC in proliferative responses of T lymphocytes to processed basic protein; (b) processed basic protein, irrespective of the genotype of APC from which it was prepared, was fully reconstituted by all IPM genotypes tested. Hence, the interaction between processed antigen and IPM was not found to be MHC-restricted. The possible implication of this conclusion is discussed.
AB - Antigen fragments, biologically degraded by antigen-presenting cells (APC), combine with Ia positive moieties (IPM) to stimulate antigen-specific T lymphocyte lines. The main objective of this study was to evaluate whether this interaction was determined by the major histocompatibility complex (MHC) genotype of the APC, thus genetically restricting antigen-specific T lymphocyte proliferative responses. To do so, we assayed the capacity of processed basic protein, associated with IPM, to stimulate basic protein specific T lymphocyte lines derived from the Lewis (LW), Brown Norway (BN), and (LW x BN)F1 rat strains. Our findings are that: (a) IPM replaced the requirement for intact APC in proliferative responses of T lymphocytes to processed basic protein; (b) processed basic protein, irrespective of the genotype of APC from which it was prepared, was fully reconstituted by all IPM genotypes tested. Hence, the interaction between processed antigen and IPM was not found to be MHC-restricted. The possible implication of this conclusion is discussed.
UR - http://www.scopus.com/inward/record.url?scp=0024340492&partnerID=8YFLogxK
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C2 - 2787050
AN - SCOPUS:0024340492
SN - 0300-9475
VL - 29
SP - 747
EP - 750
JO - Scandinavian Journal of Immunology
JF - Scandinavian Journal of Immunology
IS - 6
ER -