TY - JOUR
T1 - Absorption, translocation and metabolism of metamitron in Chenopodium album
AU - Aper, Jonas
AU - Mechant, Els
AU - Rubin, Baruch
AU - Heyerick, Arne
AU - Callebaut, Gert
AU - Mangelinckx, Sven
AU - Deforce, Dieter
AU - De Kimpe, Norbert
AU - Bulcke, Robert
AU - Reheul, Dirk
PY - 2012/2
Y1 - 2012/2
N2 - Background: In recent years, common lambsquarters (Chenopodium album L.) populations from sugar beet fields in different European countries have responded as resistant to the as-triazinone metamitron. The populations have been found to have the same D1 point mutation as known for atrazine-resistant biotypes (Ser 264 to Gly). However, pot experiments revealed that metamitron resistance is not as clear-cut as observed with triazine resistance in the past. The objectives of this study were to clarify the absorption, translocation and metabolic fate of metamitron in C. album. Results: Root absorption and foliar absorption experiments showed minor differences in absorption, translocation and metabolism of metamitron between the susceptible and resistant C. album populations. A rapid metabolism in the C. album populations was observed when metamitron was absorbed by the roots. The primary products of metamitron metabolism were identified as deamino-metamitron and metamitron-N-glucoside. PABA, known to inhibit the deamination of metribuzin, did not alter the metabolism of metamitron, and nor did the cytochrome P450 inhibitor PBO. However, inhibition of metamitron metabolism in the presence of the cytochrome P450 inhibitor ABT was demonstrated. Conclusion: Metamitron metabolism in C. album may act as a basic tolerance mechanism, which can be important in circumstances favouring this degradation pathway.
AB - Background: In recent years, common lambsquarters (Chenopodium album L.) populations from sugar beet fields in different European countries have responded as resistant to the as-triazinone metamitron. The populations have been found to have the same D1 point mutation as known for atrazine-resistant biotypes (Ser 264 to Gly). However, pot experiments revealed that metamitron resistance is not as clear-cut as observed with triazine resistance in the past. The objectives of this study were to clarify the absorption, translocation and metabolic fate of metamitron in C. album. Results: Root absorption and foliar absorption experiments showed minor differences in absorption, translocation and metabolism of metamitron between the susceptible and resistant C. album populations. A rapid metabolism in the C. album populations was observed when metamitron was absorbed by the roots. The primary products of metamitron metabolism were identified as deamino-metamitron and metamitron-N-glucoside. PABA, known to inhibit the deamination of metribuzin, did not alter the metabolism of metamitron, and nor did the cytochrome P450 inhibitor PBO. However, inhibition of metamitron metabolism in the presence of the cytochrome P450 inhibitor ABT was demonstrated. Conclusion: Metamitron metabolism in C. album may act as a basic tolerance mechanism, which can be important in circumstances favouring this degradation pathway.
KW - Common lambsquarters
KW - Herbicide resistance
KW - Herbicide tolerance
KW - Photosystem II
KW - Triazines
KW - Triazinones
UR - http://www.scopus.com/inward/record.url?scp=84855357470&partnerID=8YFLogxK
U2 - 10.1002/ps.2246
DO - 10.1002/ps.2246
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C2 - 21800410
AN - SCOPUS:84855357470
SN - 1526-498X
VL - 68
SP - 209
EP - 216
JO - Pest Management Science
JF - Pest Management Science
IS - 2
ER -