Acetylcholine and cAMP in adrenal medulla: Indirect effect

P. Boonyaviroj; Y. Gutman

doi:10.1007/BF00509267

Acetylcholine and cAMP in adrenal medulla: Indirect effect

P. Boonyaviroj, Y. Gutman^*

^*Corresponding author for this work

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Catecholamine release and cAMP accumulation were studied in bovine adrenal medulla slices in vitro. Acetylcholine (10^-4 M) and salbutamol (10^-6 M) caused increased release of catecholamines and accumulation of cAMP. Incubation in Ca²⁺-free medium abolished the release of catecholamines and the increase of cAMP caused by acetylcholine but not that caused by salbutamol. In a membrane fraction of adrenal medulla acetylcholine (10^-4 M) had no effect on adenylate cyclase activity but salbutamol (10^-6 M) caused substantial activation of adenylate cyclase. It is suggested that acetylcholine has no direct effect on adenylate cyclase or cAMP in adrenal medulla and the accumulation of cAMP observed in slices incubated with acetylcholine is due to the effect of catecholamines, released by acetylcholine, on the medullary cells.

Original language	English
Pages (from-to)	241-243
Number of pages	3
Journal	Naunyn-Schmiedeberg's Archives of Pharmacology
Volume	297
Issue number	3
DOIs	https://doi.org/10.1007/BF00509267
State	Published - Apr 1977

Keywords

Acetylcholine
Adrenal medulla
cAMP
β-receptors

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10.1007/BF00509267

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AB - Catecholamine release and cAMP accumulation were studied in bovine adrenal medulla slices in vitro. Acetylcholine (10-4 M) and salbutamol (10-6 M) caused increased release of catecholamines and accumulation of cAMP. Incubation in Ca2+-free medium abolished the release of catecholamines and the increase of cAMP caused by acetylcholine but not that caused by salbutamol. In a membrane fraction of adrenal medulla acetylcholine (10-4 M) had no effect on adenylate cyclase activity but salbutamol (10-6 M) caused substantial activation of adenylate cyclase. It is suggested that acetylcholine has no direct effect on adenylate cyclase or cAMP in adrenal medulla and the accumulation of cAMP observed in slices incubated with acetylcholine is due to the effect of catecholamines, released by acetylcholine, on the medullary cells.

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Acetylcholine and cAMP in adrenal medulla: Indirect effect

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