Acquisition of host cell genetic information by avian sarcoma virus rescued from rat cells transformed by B77 virus

M. Kotler; Sarah Salmon; U. Olshevsky

doi:10.1007/BF01311217

Acquisition of host cell genetic information by avian sarcoma virus rescued from rat cells transformed by B77 virus

M. Kotler^*, Sarah Salmon, U. Olshevsky

^*Corresponding author for this work

Institute for Medical Research Israel-Canada (IMRIC)

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The properties of avian sarcoma virus B77 grown in chick cells were compared with those of virus obtained by fusion of rat cells transformed by B77 virus (RB77 cells) with chick cells (FB77 virus). Differences in the B77 and FB77 viral genomes were demonstrated by the fingerprint technique. The rescued FB77 virus contained sequences homologous to the normal cell genome. These results suggest that the rescued FB77 virus is a recombinant between B77 virus and rat genomic elements. The RNAs from B77 and FB77 viruses were found to have similar molecular weights. The migration rates of the structural proteins and the large glycoprotein (gp85) in polyacrylamide gels were the same for the B77 and FB77 viruses, but the small glycoprotein (gp) of the FB77 virus was found to be slightly larger than the gp37 of the B77 virus.

Original language	English
Pages (from-to)	249-264
Number of pages	16
Journal	Archives of Virology
Volume	80
Issue number	4
DOIs	https://doi.org/10.1007/BF01311217
State	Published - Dec 1984

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1007/BF01311217

Cite this

@article{1b5c608fb874453bb9c39fa08b944156,

title = "Acquisition of host cell genetic information by avian sarcoma virus rescued from rat cells transformed by B77 virus",

abstract = "The properties of avian sarcoma virus B77 grown in chick cells were compared with those of virus obtained by fusion of rat cells transformed by B77 virus (RB77 cells) with chick cells (FB77 virus). Differences in the B77 and FB77 viral genomes were demonstrated by the fingerprint technique. The rescued FB77 virus contained sequences homologous to the normal cell genome. These results suggest that the rescued FB77 virus is a recombinant between B77 virus and rat genomic elements. The RNAs from B77 and FB77 viruses were found to have similar molecular weights. The migration rates of the structural proteins and the large glycoprotein (gp85) in polyacrylamide gels were the same for the B77 and FB77 viruses, but the small glycoprotein (gp) of the FB77 virus was found to be slightly larger than the gp37 of the B77 virus.",

author = "M. Kotler and Sarah Salmon and U. Olshevsky",

year = "1984",

month = dec,

doi = "10.1007/BF01311217",

language = "אנגלית",

volume = "80",

pages = "249--264",

journal = "Archives of Virology",

issn = "0304-8608",

publisher = "Springer",

number = "4",

}

TY - JOUR

T1 - Acquisition of host cell genetic information by avian sarcoma virus rescued from rat cells transformed by B77 virus

AU - Kotler, M.

AU - Salmon, Sarah

AU - Olshevsky, U.

PY - 1984/12

Y1 - 1984/12

N2 - The properties of avian sarcoma virus B77 grown in chick cells were compared with those of virus obtained by fusion of rat cells transformed by B77 virus (RB77 cells) with chick cells (FB77 virus). Differences in the B77 and FB77 viral genomes were demonstrated by the fingerprint technique. The rescued FB77 virus contained sequences homologous to the normal cell genome. These results suggest that the rescued FB77 virus is a recombinant between B77 virus and rat genomic elements. The RNAs from B77 and FB77 viruses were found to have similar molecular weights. The migration rates of the structural proteins and the large glycoprotein (gp85) in polyacrylamide gels were the same for the B77 and FB77 viruses, but the small glycoprotein (gp) of the FB77 virus was found to be slightly larger than the gp37 of the B77 virus.

AB - The properties of avian sarcoma virus B77 grown in chick cells were compared with those of virus obtained by fusion of rat cells transformed by B77 virus (RB77 cells) with chick cells (FB77 virus). Differences in the B77 and FB77 viral genomes were demonstrated by the fingerprint technique. The rescued FB77 virus contained sequences homologous to the normal cell genome. These results suggest that the rescued FB77 virus is a recombinant between B77 virus and rat genomic elements. The RNAs from B77 and FB77 viruses were found to have similar molecular weights. The migration rates of the structural proteins and the large glycoprotein (gp85) in polyacrylamide gels were the same for the B77 and FB77 viruses, but the small glycoprotein (gp) of the FB77 virus was found to be slightly larger than the gp37 of the B77 virus.

UR - http://www.scopus.com/inward/record.url?scp=0021355577&partnerID=8YFLogxK

U2 - 10.1007/BF01311217

DO - 10.1007/BF01311217

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

C2 - 6329138

AN - SCOPUS:0021355577

SN - 0304-8608

VL - 80

SP - 249

EP - 264

JO - Archives of Virology

JF - Archives of Virology

IS - 4

ER -

Acquisition of host cell genetic information by avian sarcoma virus rescued from rat cells transformed by B77 virus

Abstract

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this