Acridine orange induces translocation of phosphatidylserine to red blood cell surface

Alexander Koshkaryev, Saul Yedgar*, Hanna Relevy, Eithan Fibach, Gregory Barshtein

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


Clustering of band-3 on red blood cell (RBC) surface has been assumed to catalyze RBC phagocytosis. In studying this subject, acridine orange (AO) has commonly been employed on the assumption that it specifically induces band-3 clustering. In the present study, we show that AO strongly induces translocation of phosphatidylserine (PS) to RBC surface. Because surface PS is well known to induce RBC intercellular interaction, these findings suggest that the use of AO as a specific inducer of band-3 clustering is questionable. It is possible that band-3 clustering and PS translocation are interdependent, and this interrelationship has yet to be explored.

Original languageAmerican English
Pages (from-to)C720-C722
JournalAmerican Journal of Physiology - Cell Physiology
Issue number3 54-3
StatePublished - 1 Sep 2003


  • Acridine orange
  • Adherence
  • Band-3
  • Erythrocytes
  • Phosphatidylserine


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