Actin, myosin and actin-binding protein in lymphoid cells from human normal and leukemic cell lines

M. M. Werber, H. Ben Bassat, A. Muhlrad

Research output: Contribution to journalArticlepeer-review

Abstract

Actin, myosin and actin-binding protein have been identified in lymphoid cell lines of Null, B or T type of normal and malignant origin. The cells were fractionated into cytosol (Fraction 1), high KCl extract (Fraction 2) and mainly membranes (Fraction 3). The distribution of the three contractile proteins and the ATPase activities of myosin and actomyosin were determined in whole cells and in the fractions. Significant differences were found in the ATPase activities of myosin and actomyosin in B lymphoblastoid normal and B-lymphoma malignant cell lines. Actomyosin-ATPase activity in the normal B line was much higher than it was in the Null, B or T malignant lines examined. These results agree with the assumption that malignant transformation is accompanied by an impairment of the action of contractile proteins that leads to a decrease in motility.

Original languageEnglish
Pages (from-to)996-1000
Number of pages5
JournalIsrael Journal of Medical Sciences
Volume18
Issue number10
StatePublished - 1982
Externally publishedYes

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