Action of leishmanial excreted factor (EF) on human lymphocyte blast transformation

M. V. LONDNER; S. FRANKENBURG; G. M. SLUTZKY; C. L. GREENBLATT

doi:10.1111/j.1365-3024.1983.tb00741.x

Action of leishmanial excreted factor (EF) on human lymphocyte blast transformation

M. V. LONDNER^*, S. FRANKENBURG, G. M. SLUTZKY, C. L. GREENBLATT

^*Corresponding author for this work

Institute for Medical Research Israel-Canada (IMRIC)

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Summary. The effect of Leishmania tropica major excreted factor (EF) on human immune and normal mononuclear peripheral blood cells has been studied. The response of lymphocytes to stimulation either specifically with leishmanial antigens or non‐specifically with phytohemagglutinin (PHA) in the presence of EF was tested by the uptake of ³[H]thymidine. The results showed that EF inhibits the response of cells from immune donors to leishmanial antigens and from normal donors to PHA or PPD. Adherent and non‐adherent cells were separated and the effect of EF on both populations was analysed. The results showed that EF inhibited blast transformation if both EF and antigen were presented to each of the separate populations. The inhibition of the adherent cells (mainly monocytes) was more marked than the inhibition of the nonadherent population (mainly lymphocytes).

Original language	English
Pages (from-to)	249-256
Number of pages	8
Journal	Parasite Immunology
Volume	5
Issue number	3
DOIs	https://doi.org/10.1111/j.1365-3024.1983.tb00741.x
State	Published - May 1983

Keywords

blast transformation
excreted factor
leishmania
lymphocyte

Access to Document

10.1111/j.1365-3024.1983.tb00741.x

Cite this

@article{4837faa08b374065b9c9a17086589244,

title = "Action of leishmanial excreted factor (EF) on human lymphocyte blast transformation",

abstract = "Summary. The effect of Leishmania tropica major excreted factor (EF) on human immune and normal mononuclear peripheral blood cells has been studied. The response of lymphocytes to stimulation either specifically with leishmanial antigens or non‐specifically with phytohemagglutinin (PHA) in the presence of EF was tested by the uptake of 3[H]thymidine. The results showed that EF inhibits the response of cells from immune donors to leishmanial antigens and from normal donors to PHA or PPD. Adherent and non‐adherent cells were separated and the effect of EF on both populations was analysed. The results showed that EF inhibited blast transformation if both EF and antigen were presented to each of the separate populations. The inhibition of the adherent cells (mainly monocytes) was more marked than the inhibition of the nonadherent population (mainly lymphocytes).",

keywords = "blast transformation, excreted factor, leishmania, lymphocyte",

author = "LONDNER, {M. V.} and S. FRANKENBURG and SLUTZKY, {G. M.} and GREENBLATT, {C. L.}",

year = "1983",

month = may,

doi = "10.1111/j.1365-3024.1983.tb00741.x",

language = "אנגלית",

volume = "5",

pages = "249--256",

journal = "Parasite Immunology",

issn = "0141-9838",

publisher = "Wiley-Blackwell Publishing Ltd",

number = "3",

}

TY - JOUR

T1 - Action of leishmanial excreted factor (EF) on human lymphocyte blast transformation

AU - LONDNER, M. V.

AU - FRANKENBURG, S.

AU - SLUTZKY, G. M.

AU - GREENBLATT, C. L.

PY - 1983/5

Y1 - 1983/5

N2 - Summary. The effect of Leishmania tropica major excreted factor (EF) on human immune and normal mononuclear peripheral blood cells has been studied. The response of lymphocytes to stimulation either specifically with leishmanial antigens or non‐specifically with phytohemagglutinin (PHA) in the presence of EF was tested by the uptake of 3[H]thymidine. The results showed that EF inhibits the response of cells from immune donors to leishmanial antigens and from normal donors to PHA or PPD. Adherent and non‐adherent cells were separated and the effect of EF on both populations was analysed. The results showed that EF inhibited blast transformation if both EF and antigen were presented to each of the separate populations. The inhibition of the adherent cells (mainly monocytes) was more marked than the inhibition of the nonadherent population (mainly lymphocytes).

AB - Summary. The effect of Leishmania tropica major excreted factor (EF) on human immune and normal mononuclear peripheral blood cells has been studied. The response of lymphocytes to stimulation either specifically with leishmanial antigens or non‐specifically with phytohemagglutinin (PHA) in the presence of EF was tested by the uptake of 3[H]thymidine. The results showed that EF inhibits the response of cells from immune donors to leishmanial antigens and from normal donors to PHA or PPD. Adherent and non‐adherent cells were separated and the effect of EF on both populations was analysed. The results showed that EF inhibited blast transformation if both EF and antigen were presented to each of the separate populations. The inhibition of the adherent cells (mainly monocytes) was more marked than the inhibition of the nonadherent population (mainly lymphocytes).

KW - blast transformation

KW - excreted factor

KW - leishmania

KW - lymphocyte

UR - http://www.scopus.com/inward/record.url?scp=0020608838&partnerID=8YFLogxK

U2 - 10.1111/j.1365-3024.1983.tb00741.x

DO - 10.1111/j.1365-3024.1983.tb00741.x

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

C2 - 6866562

AN - SCOPUS:0020608838

SN - 0141-9838

VL - 5

SP - 249

EP - 256

JO - Parasite Immunology

JF - Parasite Immunology

IS - 3

ER -

Action of leishmanial excreted factor (EF) on human lymphocyte blast transformation

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this