Abstract
Mouse E-mast cells were differentiated and grown by culturing bone marrow cells in medium containing WEHI-3-conditioned medium. These cells possess surface receptors to the following agglutinins: peanut (PNA), concanavalin A (Con A), and soybean (Sb). One to 200 μg of PNA/106 E-mast cells selectively stimulated the generation of leukotriene C4 (LTC4) in the absence of β-hexosaminidase release. Exposure of 106 E-mast cells to 1 to 200 μg Con A or Sb had no effect either on preformed mediator release or on the generation of leukotrienes. LTC4 was quantitated by integrated UV absorbance after resolution by reverse phase high pressure liquid chromatography. The optimum release of LTC4 (13.2 ng/106 cells) was achieved by 50 μg of PNA/106 cells. The response is characterized by the inhibition by excess amounts of PNA. The amount of LTC4 generated during optimal PNA stimulation is lower than the amount produced after stimulation by IgE-antigen or by calcium ionophore A23187 (19.8 ng and 148 ng; respectively). The release of LTC4 began within 5 min after PNA stimulation, and reached a plateau within 45 to 60 min at 37°C. This kinetic pattern is similar to that observed after calcium ionophore A23187 stimulation of these cells. The results suggest that PNA is capable of selectively activating the 5-lipoxygenation of arachidonic acid without affecting β-hexosaminidase secretion. Apparently, separate biochemical events may serve to mobilize each class of mediators.
Original language | English |
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Pages (from-to) | 1142-1145 |
Number of pages | 4 |
Journal | Journal of Immunology |
Volume | 134 |
Issue number | 2 |
State | Published - 1985 |