Active genes are sensitive to deoxyribonuclease I during metaphase

Bruria Gazit; Howard Cedar; Israela Lerer; Ruth Voss

doi:10.1126/science.6283640

Active genes are sensitive to deoxyribonuclease I during metaphase

Bruria Gazit^*, Howard Cedar, Israela Lerer, Ruth Voss

^*Corresponding author for this work

Institute for Medical Research Israel-Canada (IMRIC)

Research output: Contribution to journal › Article › peer-review

67 Scopus citations

Abstract

The active exogenous murine leukemia virus sequences of mouse cells growing in culture are preferentially digested by deoxyribonuclease I in metaphase chromosomes. As determined by nuclear nick translation, all of the gene sequences of these cells active during interphase are in a deoxyribonuclease I-sensitive conformation during metaphase. This method of nick translation can therefore be used to label chromosomes in situ in order to visualize the active regions of the genome.

Original language	English
Pages (from-to)	648-650
Number of pages	3
Journal	Science
Volume	217
Issue number	4560
DOIs	https://doi.org/10.1126/science.6283640
State	Published - 1982

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title = "Active genes are sensitive to deoxyribonuclease I during metaphase",

abstract = "The active exogenous murine leukemia virus sequences of mouse cells growing in culture are preferentially digested by deoxyribonuclease I in metaphase chromosomes. As determined by nuclear nick translation, all of the gene sequences of these cells active during interphase are in a deoxyribonuclease I-sensitive conformation during metaphase. This method of nick translation can therefore be used to label chromosomes in situ in order to visualize the active regions of the genome.",

author = "Bruria Gazit and Howard Cedar and Israela Lerer and Ruth Voss",

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N2 - The active exogenous murine leukemia virus sequences of mouse cells growing in culture are preferentially digested by deoxyribonuclease I in metaphase chromosomes. As determined by nuclear nick translation, all of the gene sequences of these cells active during interphase are in a deoxyribonuclease I-sensitive conformation during metaphase. This method of nick translation can therefore be used to label chromosomes in situ in order to visualize the active regions of the genome.

AB - The active exogenous murine leukemia virus sequences of mouse cells growing in culture are preferentially digested by deoxyribonuclease I in metaphase chromosomes. As determined by nuclear nick translation, all of the gene sequences of these cells active during interphase are in a deoxyribonuclease I-sensitive conformation during metaphase. This method of nick translation can therefore be used to label chromosomes in situ in order to visualize the active regions of the genome.

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Active genes are sensitive to deoxyribonuclease I during metaphase

Abstract

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