Biofilm-forming Bacillus species are often involved in contamination of dairy products and therefore present a major microbiological challenge in the field of food quality and safety. In this study, we sequenced and analyzed the genomes of milk- and non-milk-derived Bacillus strains, and evaluated their biofilm-formation potential in milk. Unlike non-dairy Bacillus isolates, the dairy-associated Bacillus strains were characterized by formation of robust submerged and air–liquid interface biofilm (pellicle) during growth in milk. Moreover, genome comparison analysis revealed notable differences in putative biofilm-associated determinants between the dairy and non-dairy Bacillus isolates, which correlated with biofilm phenotype. These results suggest that biofilm formation by Bacillus species might represent a presumable adaptation strategy to the dairy environment.
Bibliographical noteFunding Information:
This work was partially supported by the Israel Dairy Board [grant number 4210343 ] grant. IO is recipient of PhD scholarship from the Ministry of Absorption and Integration of Israel as well as excellence in research scholarship from Israel Dairy Board Fund.
© 2019 The Authors
- Bacillus species
- Dairy biofilm
- Genome comparison analysis
- Milk quality
- Pellicle formation