Adaptation of Bacillus species to dairy associated environment facilitates their biofilm forming ability

Ievgeniia Ostrov; Noa Sela; Eduard Belausov; Doron Steinberg; Moshe Shemesh

doi:10.1016/j.fm.2019.02.015

Adaptation of Bacillus species to dairy associated environment facilitates their biofilm forming ability

Ievgeniia Ostrov, Noa Sela, Eduard Belausov, Doron Steinberg, Moshe Shemesh^*

^*Corresponding author for this work

Institute of Biomedical Research at the Faculty of Dental Medicine

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

Biofilm-forming Bacillus species are often involved in contamination of dairy products and therefore present a major microbiological challenge in the field of food quality and safety. In this study, we sequenced and analyzed the genomes of milk- and non-milk-derived Bacillus strains, and evaluated their biofilm-formation potential in milk. Unlike non-dairy Bacillus isolates, the dairy-associated Bacillus strains were characterized by formation of robust submerged and air–liquid interface biofilm (pellicle) during growth in milk. Moreover, genome comparison analysis revealed notable differences in putative biofilm-associated determinants between the dairy and non-dairy Bacillus isolates, which correlated with biofilm phenotype. These results suggest that biofilm formation by Bacillus species might represent a presumable adaptation strategy to the dairy environment.

Original language	English
Pages (from-to)	316-324
Number of pages	9
Journal	Food Microbiology
Volume	82
DOIs	https://doi.org/10.1016/j.fm.2019.02.015
State	Published - Sep 2019

Bibliographical note

Funding Information:
This work was partially supported by the Israel Dairy Board [grant number 4210343 ] grant. IO is recipient of PhD scholarship from the Ministry of Absorption and Integration of Israel as well as excellence in research scholarship from Israel Dairy Board Fund.

Publisher Copyright:
© 2019 The Authors

Keywords

Bacillus species
Dairy biofilm
Genome comparison analysis
Milk quality
Pellicle formation

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10.1016/j.fm.2019.02.015

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abstract = "Biofilm-forming Bacillus species are often involved in contamination of dairy products and therefore present a major microbiological challenge in the field of food quality and safety. In this study, we sequenced and analyzed the genomes of milk- and non-milk-derived Bacillus strains, and evaluated their biofilm-formation potential in milk. Unlike non-dairy Bacillus isolates, the dairy-associated Bacillus strains were characterized by formation of robust submerged and air–liquid interface biofilm (pellicle) during growth in milk. Moreover, genome comparison analysis revealed notable differences in putative biofilm-associated determinants between the dairy and non-dairy Bacillus isolates, which correlated with biofilm phenotype. These results suggest that biofilm formation by Bacillus species might represent a presumable adaptation strategy to the dairy environment.",

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AU - Shemesh, Moshe

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Adaptation of Bacillus species to dairy associated environment facilitates their biofilm forming ability

Abstract

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Keywords

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