Adenylyl cyclase activity of the fission yeast Schizosaccharomyces pombe is not regulated by guanyl nucleotides

David Engelberg, Enrique Poradosu, Giora Simchen, Alexander Levitzki*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

The adenylyl cyclase activity of the fission yeast Schizosaccharomyces pombe is localized to the plasma membrane of the cell. The enzyme utilizes Mn2+/ATP as substrate and free Mn2+ ions as an effector. Unlike the baker yeast Saccharomyces cerevisiae, S. pombe adenylyl cyclase does not utilize Mg2+/ATP as substrate and the activity is not stimulated by guanyl nucleotides. The optimal pH for the S. pombe adenylyl cyclase activity is 6.0. The activity dependence on ATP is cooperative with a Hill coefficient of 1.68 ± 0.14.

Original languageAmerican English
Pages (from-to)413-418
Number of pages6
JournalFEBS Letters
Volume261
Issue number2
DOIs
StatePublished - 26 Feb 1990

Keywords

  • Adenylyl cyclase
  • Schizosaccharomyces pombe
  • Yeast

Fingerprint

Dive into the research topics of 'Adenylyl cyclase activity of the fission yeast Schizosaccharomyces pombe is not regulated by guanyl nucleotides'. Together they form a unique fingerprint.

Cite this