Adenylyl cyclase activity of the fission yeast Schizosaccharomyces pombe is not regulated by guanyl nucleotides

David Engelberg; Enrique Poradosu; Giora Simchen; Alexander Levitzki

doi:10.1016/0014-5793(90)80605-I

Adenylyl cyclase activity of the fission yeast Schizosaccharomyces pombe is not regulated by guanyl nucleotides

David Engelberg
, Enrique Poradosu
, Giora Simchen
, Alexander Levitzki^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

The adenylyl cyclase activity of the fission yeast Schizosaccharomyces pombe is localized to the plasma membrane of the cell. The enzyme utilizes Mn²⁺/ATP as substrate and free Mn²⁺ ions as an effector. Unlike the baker yeast Saccharomyces cerevisiae, S. pombe adenylyl cyclase does not utilize Mg²⁺/ATP as substrate and the activity is not stimulated by guanyl nucleotides. The optimal pH for the S. pombe adenylyl cyclase activity is 6.0. The activity dependence on ATP is cooperative with a Hill coefficient of 1.68 ± 0.14.

Original language	English
Pages (from-to)	413-418
Number of pages	6
Journal	FEBS Letters
Volume	261
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(90)80605-I
State	Published - 26 Feb 1990

Keywords

Adenylyl cyclase
Schizosaccharomyces pombe
Yeast

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10.1016/0014-5793(90)80605-I

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title = "Adenylyl cyclase activity of the fission yeast Schizosaccharomyces pombe is not regulated by guanyl nucleotides",

abstract = "The adenylyl cyclase activity of the fission yeast Schizosaccharomyces pombe is localized to the plasma membrane of the cell. The enzyme utilizes Mn2+/ATP as substrate and free Mn2+ ions as an effector. Unlike the baker yeast Saccharomyces cerevisiae, S. pombe adenylyl cyclase does not utilize Mg2+/ATP as substrate and the activity is not stimulated by guanyl nucleotides. The optimal pH for the S. pombe adenylyl cyclase activity is 6.0. The activity dependence on ATP is cooperative with a Hill coefficient of 1.68 ± 0.14.",

keywords = "Adenylyl cyclase, Schizosaccharomyces pombe, Yeast",

author = "David Engelberg and Enrique Poradosu and Giora Simchen and Alexander Levitzki",

year = "1990",

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doi = "10.1016/0014-5793(90)80605-I",

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T1 - Adenylyl cyclase activity of the fission yeast Schizosaccharomyces pombe is not regulated by guanyl nucleotides

AU - Engelberg, David

AU - Poradosu, Enrique

AU - Simchen, Giora

AU - Levitzki, Alexander

PY - 1990/2/26

Y1 - 1990/2/26

N2 - The adenylyl cyclase activity of the fission yeast Schizosaccharomyces pombe is localized to the plasma membrane of the cell. The enzyme utilizes Mn2+/ATP as substrate and free Mn2+ ions as an effector. Unlike the baker yeast Saccharomyces cerevisiae, S. pombe adenylyl cyclase does not utilize Mg2+/ATP as substrate and the activity is not stimulated by guanyl nucleotides. The optimal pH for the S. pombe adenylyl cyclase activity is 6.0. The activity dependence on ATP is cooperative with a Hill coefficient of 1.68 ± 0.14.

AB - The adenylyl cyclase activity of the fission yeast Schizosaccharomyces pombe is localized to the plasma membrane of the cell. The enzyme utilizes Mn2+/ATP as substrate and free Mn2+ ions as an effector. Unlike the baker yeast Saccharomyces cerevisiae, S. pombe adenylyl cyclase does not utilize Mg2+/ATP as substrate and the activity is not stimulated by guanyl nucleotides. The optimal pH for the S. pombe adenylyl cyclase activity is 6.0. The activity dependence on ATP is cooperative with a Hill coefficient of 1.68 ± 0.14.

KW - Adenylyl cyclase

KW - Schizosaccharomyces pombe

KW - Yeast

UR - https://www.scopus.com/pages/publications/0025057465

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AN - SCOPUS:0025057465

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VL - 261

SP - 413

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JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

Adenylyl cyclase activity of the fission yeast Schizosaccharomyces pombe is not regulated by guanyl nucleotides

Abstract

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