Adhering interacting cells to two opposing coverslips allows super-resolution imaging of cell-cell interfaces

Julia Sajman, Yair Razvag, Shachar Schidorsky, Seon Kinrot, Kobi Hermon, Oren Yakovian, Eilon Sherman*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Cell-cell interfaces convey mechanical and chemical information in multicellular systems. Microscopy has revealed intricate structure of such interfaces, yet typically with limited resolution due to diffraction and unfavourable orthogonal orientation of the interface to the coverslip. We present a simple and robust way to align cell-cell interfaces in parallel to the coverslip by adhering the interacting cells to two opposing coverslips. We demonstrate high-quality diffraction-limited and super-resolution imaging of interfaces (immune-synapses) between fixed and live CD8+ T-cells and either antigen presenting cells or melanoma cells. Imaging methods include bright-field, confocal, STED, dSTORM, SOFI, SRRF and large-scale tiled images. The low background, lack of aberrations and enhanced spatial stability of our method relative to existing cell-trapping techniques allow use of these methods. We expect that the simplicity and wide-compatibility of our approach will allow its wide dissemination for super-resolving the intricate structure and molecular organization in a variety of cell-cell interfaces.

Original languageAmerican English
Article number439
JournalCommunications Biology
Volume4
Issue number1
DOIs
StatePublished - 1 Apr 2021

Bibliographical note

Publisher Copyright:
© 2021, The Author(s).

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