Adjustment to low light intensity enhances susceptibility of bean leaves to oxidative stress

Oren Shainberg*, Baruch Rubin, Haim D. Rabinowitch, Yael Libal-Weksler, Elisha Tel-Or

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

We studied the effect of a short term shading on the activity of the antioxidative system in primary leaves of Phasedus vulgaris (cv. Pinto) and the response of the shaded beans to subsequent oxidative stress. Shaded bean leaves demonstrated a 35 % decrease in activity and concentration of glutathione reductase (GR, EC 1.6.4.2) and reduced glutathione (GSH) and a 30 % decrease in concentration of chloroplastic CuZn-superoxide dismutase (SOD, EC 1.15.11). No difference was observed in activities of ascorbate peroxidase (APX, EC 1.11.1.11) and catalase (EC 1.11.1.6) and the concentration of cytosolic CuZn-SOD. Two days shading (20 % of that of daylight) of bean plants resulted in increased sensitivity to methyl viologen and SO2 as compared to unshaded control plants but there was no difference in the sensitivity to ozone. Shaded leaves suffered marked increase in ion leakage and water losses, and developed more necrotic lesions as compared to the control leaves. Our results show that in beans, even a short transition of plants from high to low light intensity, decreases the activity of chloroplastic antioxidative mechanisms that are needed to counteract subsequent oxidative stress.

Original languageEnglish
Pages (from-to)393-398
Number of pages6
JournalJournal of Plant Physiology
Volume155
Issue number3
DOIs
StatePublished - Sep 1999

Keywords

  • Beans
  • Glutathione
  • Glutathione Reductase
  • Light intensity
  • Methyl viologen
  • Oxidative stress
  • Phaseolus vulgaris
  • SO
  • Superoxide dismutase

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